BRC No.RBRC00891
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Spen<tm1Hon>/HonRbrc
Former Common nameMINT-KO (C57BL/6 background)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Strain developmentDeveloped by Dr. Tasuku Honjo, Kyoto University in 2003. A pgk-neo was transfered into E14 ES cells to replace nucleotide 4891 of the coding region of Mint. The Mint deficient mice were backcrossed to C57BL/6 for 16 times.
Strain descriptionB6.129P2-Mint-pending<tm1Hon>/HonRbrc. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Homozygous mutant mice are embryonic lethal due to developmental abnormality of heart, spleen and liver. This strain is useful for elucidating of Notch/RBP-J signaling pathway mechanism in mammals.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)The reproductive performance is good. Homozygous mutant mice are embryonic lethal.
ReferencesImmunity, 18, 301-312 (2003). 12594956

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
SpenMsx2-interacting nuclear target protein (MINT)4Spen<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse MINT genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
Research applicationDevelopmental Biology Research
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Immunity 18, 301-312 (2003).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data