|Former Common name||B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01|
|ES Cell line|
|Background strain||B6C3F1[C57BL/6N and C3H/HeN]|
|Appearance||black [a/a B/B C/C]|
|Strain development||Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. Acr3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mice were crossed to C57BL/6.|
|Strain description||Transgenic mice expressing GFP in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter.|
|Colony maintenance||Carrier x Carrier (Homozygote x Homozygote)|
|References||FEBS Lett., 449, 277-283 (1999). 10338148|
|Examination Date / Room / Rack||2018/12/17Room:3-BRack:G|
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide
|Donor DNA||mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal|
|Research application||Fluorescent Proteins/lacZ System|
|Specific Term and Conditions||The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 449, 277-283 (1999). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.|
|Depositor||Masaru Okabe (Osaka University)|
|Strain Status||Frozen embryos|
|Strain Availability||Recovered litters from cryopreserved embryos (2 to 4 months)|
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
|Additional Info.||Genotyping protocol -PCR-|
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
BRC mice in Publications
Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
Biochem. Biophys. Res. Commun. (2018) 29705697
Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T.
In vitro murine spermatogenesis in an organ culture system.
Biol. Reprod. 83(2) 261-7(2010) 20393168
Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T.
In vitro production of fertile sperm from murine spermatogonial stem cell lines.
Nat Commun 2 472(2011) 21915114
Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T.
In vitro production of functional sperm in cultured neonatal mouse testes.
Nature 471(7339) 504-7(2011) 21430778
Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM.
Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis.
Radiat. Res. (2018) 29595376
Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
Sci Rep 7(1) 15459(2017) 29133858
Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.
Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device.
Sci Rep (2016) 26892171