Strain Data Sheet


Strain Information

BRC No.RBRC00886
SpeciesMus musculus
Strain nameB6;B6C3-Tg(Acro3-EGFP)01Osb
Former Common nameB6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01
H-2 Haplotype
ES Cell line
Background strainB6C3F1[C57BL/6N and C3H/HeN]
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. Acr3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionTransgenic mice expressing GFP in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter.
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote)
ReferencesFEBS Lett., 449, 277-283 (1999). 10338148

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide

Ordering Information

Donor DNAmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 449, 277-283 (1999). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697

Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T.
In vitro murine spermatogenesis in an organ culture system.
Biol Reprod 83(2) 261-7(2010) 20393168

Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S.
Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis.
Commun Biol 5(1) 504(2022) 35618762

Okugi K, Kuwahara N, Yanome N, Yamada K, Ito T, Takano A, Ohira S, Nagai A, Toné S.
An in vitro system for experimentally induced cryptorchidism.
Histochem Cell Biol 157(3) 297-307(2022) 35190876

Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM.
The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy.
J Clin Med 9(4) (2020) 32290436

Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T.
In vitro production of fertile sperm from murine spermatogonial stem cell lines.
Nat Commun 2 472(2011) 21915114

Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T.
In vitro production of functional sperm in cultured neonatal mouse testes.
Nature 471(7339) 504-7(2011) 21430778

Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM.
Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis.
Radiat Res 189(6) 661-667(2018) 29595376

Hashimoto K, Odaka H, Ishikawa-Yamauchi Y, Nagata S, Nakamura H, Kimura H, Sato T, Makiyama K, Ogawa T.
Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.
Sci Rep 13(1) 12354(2023) 37524742

Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
Sci Rep 7(1) 15459(2017) 29133858

Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.
Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device.
Sci Rep 6 21472(2016) 26892171