RBRC00869

Information1

Image
BRC No.RBRC00869
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(GOF18dPE/EGFP)48Ymat
Former Common nameB6.D2-Tg(GOF18 delta PE/EGFP)48, GOF18-delta PE-GFP line48 transgenic mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
AppearanceBlack
Strain developmentDeveloped by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Growth Differ., 41, 675-684 (1999). 10646797

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNOct 3/4 promoter

Information2

Donor DNAOct 3 promoter, Jellyfish GFP cDNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth. Differ., 41, 675-684 (1999).
DepositorYasuhisa Matsui (Research Institute, Osaka Medical Center for Maternal and Child Health)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Miki H, Lee J, Inoue K, Ogonuki N, Noguchi Y, Mochida K, Kohda T, Nagashima H, Ishino F, Ogura A.
Microinsemination with first-wave round spermatids from immature male mice.
J. Reprod. Dev. 50(1) 131-7(2004) 15007210