Strain Data Sheet


Strain Information

BRC No.RBRC00869
SpeciesMus musculus
Strain nameB6.Cg-Tg(GOF18dPE/EGFP)48Ymat
Former Common nameB6.D2-Tg(GOF18 delta PE/EGFP)48, GOF18-delta PE-GFP line48 transgenic mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDeveloped by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Growth Differ., 41, 675-684 (1999). 10646797

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPOct 3/4 promoter

Ordering Information

Donor DNAOct 3 promoter, Jellyfish GFP cDNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth. Differ., 41, 675-684 (1999).
DepositorYasuhisa Matsui (Research Institute, Osaka Medical Center for Maternal and Child Health)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Miki H, Lee J, Inoue K, Ogonuki N, Noguchi Y, Mochida K, Kohda T, Nagashima H, Ishino F, Ogura A.
Microinsemination with first-wave round spermatids from immature male mice.
J Reprod Dev 50(1) 131-7(2004) 15007210