RBRC00858

Information1

Image
BRC No.RBRC00858
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc
Former Common nameIRF-3 KO/B6; IRF-3 Knockout mice, C57BL/6J
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Tadatsugu Taniguchi, Graduate School of Medicine and Faculty of Medicine, University of Tokyo. C57BL/6 semicongenic strain.
Strain descriptionThe Irf3 deficient mice were created by replacing a 2.2 kb region of Irf3 containing a putative transcription initiation site and the coding N-terminal region of the DNA binding domain. The analysis of MEFs demonstrated loss of Irf3 protein. Homozygous null mice are more susceptible to viral infection such as encephalomyocarditis virus and exhibit lower serum interferon levels in response to viral infection, indicating incapability of the mutant to respond against viral infection.
Colony maintenanceHomozygote x Homozygote
ReferencesImmunity, 13, 539-548 (2000). 11070172

Health Report

Examination Date / Room / Rack2020/06/01Room:3-DRack:D
2020/02/17Room:3-DRack:D
2019/12/16Room:3-DRack:D
2019/10/21Room:3-DRack:D
2019/08/26Room:3-DRack:D
2019/06/17Room:3-DRack:D
2019/04/15Room:3-DRack:D
2019/02/18Room:3-DRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Irf3interferon regulatory factor 37Irf3<tm1Ttg>targeted mutation 1, Tadatsugu Taniguchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bcl2l12BCL2-like 12 (proline rich)7Bcl2l12<tm1Ttg>targeted mutation 1, Tadatsugu Taniguchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IRF-3 genomic DNA, mouse Bcl2l12 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
DepositorTadatsugu Taniguchi (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month May 2005
Genetic Background

BRC mice in Publications

Smith H, Liu XY, Dai L, Goh ET, Chan AT, Xi J, Seh CC, Qureshi IA, Lescar J, Ruedl C, Gourlay R, Morton S, Hough J, McIver EG, Cohen P, Cheung PC.
The role of TBK1 and IKK in the expression and activation of Pellino 1.
Biochem. J. 434(3) 537-48(2011) 21204785

Goriely S, Molle C, Nguyen M, Albarani V, Haddou NO, Lin R, De Wit D, Flamand V, Willems F, Goldman M.
Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation.
Blood 107(3) 1078-84(2006) 16219795

Zhang SM, Zhu LH, Li ZZ, Wang PX, Chen HZ, Guan HJ, Jiang DS, Chen K, Zhang XF, Tian S, Yang D, Zhang XD, Li H.
Interferon regulatory factor 3 protects against adverse neo-intima formation.
Cardiovasc. Res. (2014) 24596398

Dogusan Z, Garc a M, Flamez D, Alexopoulou L, Goldman M, Gysemans C, Mathieu C, Libert C, Eizirik DL, Rasschaert J.
Double-stranded RNA induces pancreatic beta-cell apoptosis by activation of the toll-like receptor 3 and interferon regulatory factor 3 pathways.
Diabetes 57(5) 1236-45(2008) 18223009

Loi P, Yuan Q, Torres D, Delbauve S, Laute MA, Lalmand MC, P tein M, Goriely S, Goldman M, Flamand V.
IRF3 deficiency leads to IL-17-mediated liver ischemia-reperfusion injury.
Hepatology (2012) 22911673

Prantner D, Sikes JD, Hennings L, Savenka AV, Basnakian AG, Nagarajan UM.
IRF3 protects from uterine horn pathology during Chlamydia muridarum genital infection in mice.
Infect. Immun. (2011) 21788382

Marichal T, Bedoret D, Mesnil C, Pichavant M, Goriely S, Trottein F, Cataldo D, Goldman M, Lekeux P, Bureau F, Desmet CJ.
Interferon response factor 3 is essential for house dust mite-induced airway allergy.
J. Allergy Clin. Immunol. 126(4) 836-844.e13(2010) 20673978

Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N.
IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide.
J. Immunol. 198(9) 3637-3649(2017) 28363903

Prantner D, Darville T, Nagarajan UM.
Stimulator of IFN gene is critical for induction of IFN-beta during Chlamydia muridarum infection.
J. Immunol. 184(5) 2551-60(2010) 20107183

Molle C, Goldman M, Goriely S.
Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
J. Immunol. 184(4) 1784-92(2010) 20083668

Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S.
IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
J. Immunol. 178(12) 7607-15(2007) 17548596

Stevens SL, Leung PY, Vartanian KB, Gopalan B, Yang T, Simon RP, Stenzel-Poore MP.
Multiple preconditioning paradigms converge on interferon regulatory factor-dependent signaling to promote tolerance to ischemic brain injury.
J. Neurosci. 31(23) 8456-63(2011) 21653850

Marsh B, Stevens SL, Packard AE, Gopalan B, Hunter B, Leung PY, Harrington CA, Stenzel-Poore MP.
Systemic lipopolysaccharide protects the brain from ischemic injury by reprogramming the response of the brain to stroke: a critical role for IRF3.
J. Neurosci. 29(31) 9839-49(2009) 19657036

Christensen JE, Fenger C, Issazadeh-Navikas S, Krug A, Liljestr m P, Goriely S, Paludan SR, Finsen B, Christensen JP, Thomsen AR.
Differential impact of interferon regulatory factor 7 in the initiation of the type I interferon response in the LCMV infected CNS versus the periphery.
J. Virol. (2012) 22514347

Zhou S, Cerny AM, Zacharia A, Fitzgerald KA, Kurt-Jones EA, Finberg RW.
Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus.
J. Virol. 84(18) 9452-62(2010) 20592086

Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y.
Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner.
J. Virol. 83(15) 7629-40(2009) 19474102

Johnson J, Molle C, Aksoy E, Goldman M, Goriely S, Willems F.
A conventional protein kinase C inhibitor targeting IRF-3-dependent genes differentially regulates IL-12 family members.
Mol. Immunol. (2011) 21550664

Warz e B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ.
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways.
Mol. Immunol. 48(1-3) 119-27(2010) 20947170

Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y.
Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system.
Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. 84(10) 439-51(2008) 19075516

Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S.
Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes.
Proc. Natl. Acad. Sci. U.S.A. 110(34) E3189-97(2013) 23918362

Kiyotani K, Sakaguchi T, Kato A, Nagai Y, Yoshida T.
Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice.
Virology 359(1) 82-91(2007) 17027894