BRC No.RBRC00857
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Treh<tm1Tkam>/Rbrc
Former Common nametrehalase Knockout mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masashi Kurimoto, Biochemical Laboratories, Inc. in 2001-2002. A neomycin selection cassette replaced exons 5-10 of the Treh gene.
Strain descriptionB6;B6CB-Treh<tm1Tkam>. Targeted disruption of the trehalase gene. The mice with this mutant allele lack the trehalase activity in small intestine and kidney.
Colony maintenanceSibling x Sibling (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
ReferencesTrehalose itself plays a critical role on lipid metabolism: Trehalose increases jejunum cytoplasmic lipid droplets which negatively correlatedwith mesenteric adipocyte size in both HFD-fed trehalase KO and WT mice.
Nutr. Res., 23, 287-298 (2003).

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trehtrehalase (brush-border membrane glycoprotein)9Treh<tm1Tkam>targeted mutation 1, Takashi Kamiya

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse trehalase genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nutrition Research, 23, 287-298 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR, Tsunetaka Ohta (tsunetaka.ota@hb.nagase.co.jp).
The material is the subject of a Japanese Patent. For non-commercial research use only. Licenses for commercial use may be obtained directly from Hayashibara Biochemical Laboratories, Inc Co. Ltd.
In case of applications for special permission that are based on the fruits of investigations using the named resources, users must hold specific conferences with the DEPOSITOR.
DepositorMasashi Kurimoto (Hayashibara Biochemical Laboratories, Inc.)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data