Strain Data Sheet

RBRC00850

Information1

Image
BRC No.RBRC00850
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Itgal<tm1Mak>
Former Common nameC57BL/6-[KO]LFA-1; B6.129P2-Itgb2<tm1Oci>
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strain
Appearanceblack
Strain developmentFrom Tak. W. Mak of ontario Cancer Institute, N6
Strain descriptionOriginal line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
Colony maintenance
ReferencesJ. Exp. Med., 183, 1415-1426 (1996). 8666900

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itgalintegrin alpha L7Itgal<tm1Mak>targeted mutation 1, Tak W MakCD11a, Integrin alpha L

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, tk poly A, mouse CD11a genomic DNA
Research applicationImmunology and Inflammation Research
Virology Research
covid19_infection
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K.
TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.
Nat Commun 8(1) 1592(2017) 29150602