RBRC00843

Information1

Image
BRC No.RBRC00843
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Maf<tm1Mym>/2Rbrc
Former Common name44A, c-Maf/2 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearance
Strain developmentDeveloped by Drs. Shimako Kawauchi and Satoru Takahashi at University of Tsukuba in 1998. Both the neomycin and lacZ gene were inserted and replacing most of the coding region of c-Maf gene.
Strain descriptionB6;129P2-Maf<tm2Mym>. c-Maf is a transcriptional factor that belongs to the large Maf family. This gene is essential for normal lens development. Homozygous mutant mice show defective differentiation of lens fiber cells, and the expression of crystalin genes was severely impaired in the mice.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)Homozygous mutant mice die within 3 days after birth.
ReferencesJ. Biol. Chem., 274, 19254-19260 (1999). 10383433

Health Report

Examination Date / Room / Rack2011/01/11Room:4-2Rack:A
2010/11/08Room:4-2Rack:A
2010/09/06Room:4-2Rack:A
2010/07/05Room:4-2Rack:A
2010/05/10Room:4-2Rack:A
2010/03/08Room:4-2Rack:A
2007/08/20Room:3-ARack:L
2007/06/18Room:3-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mafavian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog8Maf<tm2Mym>targeted mutation 2, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse c-Maf genomic DNA, E. coli neomycin phosphotransferase, E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research applicationImmunology and Inflammation Research
Sensorineural Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorSatoru Takahashi (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data