RBRC00822

Information1

Image
BRC No.RBRC00822
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(deGFP)20Imeg
Former Common nameOct-delta PE-GFP#20, TgN(deGFP)20Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Biol., 258, 209-225 (2003). 12781694

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNOct 3/4 promoter

Information2

Donor DNAOct 3/4 promoter, Jellyfish GFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKuniya Abe (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
Large, Male Germ Cell-Specific Hypomethylated DNA Domains With Unique Genomic and Epigenomic Features on the Mouse X Chromosome.
DNA Res. (2013) 23861320