Strain Data Sheet

RBRC00822

Strain Information

Image
BRC No.RBRC00822
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(deGFP)20Imeg
Former Common nameOct-delta PE-GFP#20, TgN(deGFP)20Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Identification and characterization of stem cells in prepubertal spermatogenesis in mice.
Ohbo K, Yoshida S, Ohmura M, Ohneda O, Ogawa T, Tsuchiya H, Kuwana T, Kehler J, Abe K, Scholer H R, Suda T
Dev. Biol., 258, 209-225 (2003). 12781694

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNOct 3/4 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAOct 3/4 promoter, Jellyfish GFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
    Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorKuniya Abe (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
    Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
    DNA Res 20(6) 549-65(2013) 23861320
    Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
    Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.
    Genes Cells 13(8) 863-77(2008) 18782224