Strain Information | |
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Image | |
BRC No. | RBRC00822 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(deGFP)20Imeg |
Former Common name | Oct-delta PE-GFP#20, TgN(deGFP)20Imeg |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black |
Strain development | Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822). |
Strain description | Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Ohbo K, Yoshida S, Ohmura M, Ohneda O, Ogawa T, Tsuchiya H, Kuwana T, Kehler J, Abe K, Scholer H R, Suda T Dev. Biol., 258, 209-225 (2003). 12781694 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GFP | Green Fluorescent Protein (Aequorea victoria) | UN | Oct 3/4 promoter |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Oct 3/4 promoter, Jellyfish GFP cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Kuniya Abe (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K. Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome. DNA Res 20(6) 549-65(2013) 23861320 |
Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K. Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells 13(8) 863-77(2008) 18782224 |