|Former Common name||Oct-delta PE-GFP#20, TgN(deGFP)20Imeg|
|ES Cell line|
|Strain development||Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).|
|Strain description||Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.|
|Colony maintenance||Carrier x Noncarrier [C57BL/6JJcl]|
|References||Dev. Biol., 258, 209-225 (2003). 12781694|
|Examination Date / Room / Rack||No Data|
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNOct 3/4 promoter
|Donor DNA||Oct 3/4 promoter, Jellyfish GFP cDNA|
|Research application||Fluorescent Proteins/lacZ System|
|Specific Term and Conditions||The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. |
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
|Depositor||Kuniya Abe (RIKEN)|
|Strain Status||Frozen embryos|
|Strain Availability||Recovered litters from cryopreserved embryos (2 to 4 months)|
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
|Additional Info.||GFP Transfer License (Japanese / English)|
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Genotyping protocol -PCR-
BRC mice in Publications
Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
Large, Male Germ Cell-Specific Hypomethylated DNA Domains With Unique Genomic and Epigenomic Features on the Mouse X Chromosome.
DNA Res. (2013) 23861320