Strain Data Sheet

RBRC00821

update : 2023-05-22

Information1
Image
BRC No.	RBRC00821
Type	Transgene
Species	Mus musculus
Strain name	C57BL/6J-Tg(deGFP)18Imeg
Former Common name	Oct-delta PE-GFP #18, TgN(deGFP)18Imeg
H-2 Haplotype
ES Cell line
Background strain	C57BL/6JJcl
Appearance	black
Strain development	Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).
Strain description	Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenance	Carrier x Noncarrier [C57BL/6JJcl]
References	Dev. Biol., 258, 209-225 (2003). 12781694

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Oct 3/4 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN

Information2
Donor DNA	mouse Oct 3/4 genomic DNA, Jellyfish GFP cDNA, SV40 polyA
Research application	Developmental Biology Research Fluorescent Proteins/lacZ System Reproductive Biology Research
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Depositor	Kuniya Abe (RIKEN)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month)
Additional Info.	Mouse of the Month Sep 2005 Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP Genotyping protocol -PCR-

BRC mice in Publications

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K. Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions. Cell Rep 2(5) 1363-74(2012) 23142660 Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K. Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome. DNA Res 20(6) 549-65(2013) 23861320 Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K. Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells 13(8) 863-77(2008) 18782224 Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A. Birth of mice produced by germ cell nuclear transfer. Genesis 41(2) 81-6(2005) 15712265 Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H. New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice. PLoS Genet 5(12) e1000756(2009) 19997484 Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K. Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells. Stem Cells Dev 21(8) 1239-49(2012) 21861689

BRC mice in Publications

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
Cell Rep 2(5) 1363-74(2012) 23142660

Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
DNA Res 20(6) 549-65(2013) 23861320

Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.
Genes Cells 13(8) 863-77(2008) 18782224

Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A.
Birth of mice produced by germ cell nuclear transfer.
Genesis 41(2) 81-6(2005) 15712265

Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
PLoS Genet 5(12) e1000756(2009) 19997484

Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K.
Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells.
Stem Cells Dev 21(8) 1239-49(2012) 21861689

Information1

Health Report

Gene

Information2

BRC mice in Publications