RBRC00821

Information1

Image
BRC No.RBRC00821
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(deGFP)18Imeg
Former Common nameOct-delta PE-GFP #18, TgN(deGFP)18Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Biol., 258, 209-225 (2003). 12781694

Health Report

Examination Date / Room / Rack2011/08/29Room:3-ARack:H
2011/06/20Room:3-ARack:H
2011/04/18Room:3-ARack:H
2007/12/17Room:3-BRack:F
2007/10/15Room:3-BRack:F
2007/08/20Room:3-BRack:F
2007/06/18Room:3-BRack:F
2007/04/17Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNmouse Oct 3/4 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyAUN

Information2

Donor DNAmouse Oct 3/4 genomic DNA, Jellyfish GFP cDNA, SV40 polyA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Reproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKuniya Abe (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Mouse of the Month Sep 2005

BRC mice in Publications

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
Molecular Identification of t(w5): Vps52 Promotes Pluripotential Cell Differentiation through Cell-Cell Interactions.
Cell Rep (2012) 23142660

Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
Large, Male Germ Cell-Specific Hypomethylated DNA Domains With Unique Genomic and Epigenomic Features on the Mouse X Chromosome.
DNA Res. (2013) 23861320

Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.
Genes Cells 13(8) 863-77(2008) 18782224

Hiromi MIKI, Kimiko INOUE, Takashi KOHDA, Arata HONDA, Narumi OGONUKI, Misako YUZURIHA, Nathan MISE, Yasuhisa MATSUI, Tadashi BABA, Kuniya ABE, Fumitoshi ISHINO, Atsuo OGURA
Birth of mice produced by germ cell nuclear transfer.
Genesis 41 81-86(2005) 15712265

Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
PLoS Genet. 5(12) e1000756(2009) 19997484

Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K.
Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells.
Stem Cells Dev. (2011) 21861689