RBRC00820

Information1

Image
BRC No.RBRC00820
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameTgH(qkI)2Imeg
Former Common nameqkI Knockout mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceagouti
Strain developmentDeveloped by Kuniya Abe and Ken-ichi Yamamura, Insitute of Molecular Embryology and Genetics, Kumamoto University. E14.1 ES cells were used for generation of the knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionQk (quaking) gene knockout mice. The initiation codon and first 40 amino acids of a highly conserved QUA1 domain of Qk gene were replaced with an IRES and a betageo fusion gene. Homozygous mutant mice die in utero E9.5 and E10.5.
Colony maintenanceSibling Mating (Heterozygote X Wild)Heterozygote
ReferencesDev. Growth Differ., 45, 449-462 (2003). 14706070

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Qkquaking17Qk<tm1Imeg>targeted mutation 1, Institute of Molecular Embryology and Genetics

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)17promoter less

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyAUN

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), beta-geo(E. coli lacZ, E. coli neo), SV40 poly A, mouse qkI genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Defective smooth muscle development in qkI-deficient mice.
Dev. Growth Differ., 45, 449-462 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKuniya Abe (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP

BRC mice in Publications

No Data