Strain Data Sheet
RBRC00815
Information1 | |
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| Image |  |
| BRC No. | RBRC00815 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus molossinus |
| Strain name | MSM.Cg-Trp53<tm1Sia>/JpkRbrc |
| Former Common name | MSM-p53 KO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | MSM/Ms |
| Appearance | white-bellied agouti [A<w>/A<w> B/B C/C] |
| Strain development | Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences. |
| Strain description | Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background). |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to MSM/Ms] |
| References | Oncogene, 8, 3313-3322 (1993). 7504233 Jpn. J. Cancer Res., 87, 696-701 (1996). 8698618 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA |
| Research application | Cancer Research Cell Biology Research Immunology and Inflammation Research Mouse Models for Human Disease |
| Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
| Depositor | Ryo Kominami (Niigata University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Mouse of the Month Jun 2005 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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