RBRC00808

Information1

Image
BRC No.RBRC00808
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc
Former Common nameEmx1-Cre
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack (a/a B/B C/C)
Strain developmentDeveloped by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J.
Strain descriptionEmx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk-neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNature, 406, 726-731 (2000). 10963597

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1(cre)Ito>targeted mutation 1 (cre), Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase6cremouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 406, 726-731 (2000).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month June 2006

BRC mice in Publications

Imayoshi I, Shimogori T, Ohtsuka T, Kageyama R.
Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
Development 135(15) 2531-41(2008) 18579678

Kita A, Imayoshi I, Hojo M, Kitagawa M, Kokubu H, Ohsawa R, Ohtsuka T, Kageyama R, Hashimoto N.
Hes1 and Hes5 control the progenitor pool, intermediate lobe specification, and posterior lobe formation in the pituitary development.
Mol. Endocrinol. 21(6) 1458-66(2007) 17426285

Kinameri E, Inoue T, Aruga J, Imayoshi I, Kageyama R, Shimogori T, Moore AW.
Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis.
PLoS ONE 3(12) e3859(2008) 19050759