Strain Data Sheet


Strain Information

BRC No.RBRC00806
SpeciesMus musculus
Strain nameGFP-LC3#53
Former Common nameGFP-LC3#53
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDeveloped by Noboru Mizushima, National Institute for Basic Biology. The transgene was injected into the pronuclei of C57BL/6 x (C57BL/6 x DBA/2)F1 fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express a fusion cDNA encoding enhanced GFP jointed at its C-terminus to rat microtubule-associated protein 1 light chain 3 beta under the control of the CAG promoter. The occurrence of autophagy in mouse tissues can be monitored by the GFP.
Colony maintenanceCarrier x Carrier, Noncarrier [or Crossing to C57BL/6JJcl]
ReferencesNature, 432, 1032-1036 (2004). 15525940
Mol. Biol. Cell., 15, 1101-1111 (2004). 14699058

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:A


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map1lc3bmicrotubule-associated protein 1 light (rat)2Map1lc3bmicrotubule associating protein (MAP)1 light chain 3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)2CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, rat LC3 cDNA
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell 15, 1101-1111 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-
Mouse of the Month Mar 2005
Mouse of the Month Mar 2012, Mouse of the Month Oct 2016

BRC mice in Publications

Dhingra A, Alexander D, Reyes-Reveles J, Sharp R, Boesze-Battaglia K.
Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.
Adv Exp Med Biol 1074 609-616(2018) 29721994

Kanamori H, Takemura G, Maruyama R, Goto K, Tsujimoto A, Ogino A, Li L, Kawamura I, Takeyama T, Kawaguchi T, Nagashima K, Fujiwara T, Fujiwara H, Seishima M, Minatoguchi S.
Functional significance and morphological characterization of starvation-induced autophagy in the adult heart.
Am J Pathol 174(5) 1705-14(2009) 19342365

Adhami F, Liao G, Morozov YM, Schloemer A, Schmithorst VJ, Lorenz JN, Dunn RS, Vorhees CV, Wills-Karp M, Degen JL, Davis RJ, Mizushima N, Rakic P, Dardzinski BJ, Holland SK, Sharp FR, Kuan CY.
Cerebral ischemia-hypoxia induces intravascular coagulation and autophagy.
Am J Pathol 169(2) 566-83(2006) 16877357

Koike M, Shibata M, Waguri S, Yoshimura K, Tanida I, Kominami E, Gotow T, Peters C, von Figura K, Mizushima N, Saftig P, Uchiyama Y.
Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease).
Am J Pathol 167(6) 1713-28(2005) 16314482

Nishimura F, Naruishi H, Naruishi K, Yamada T, Sasaki J, Peters C, Uchiyama Y, Murayama Y.
Cathepsin-L, a key molecule in the pathogenesis of drug-induced and I-cell disease-mediated gingival overgrowth: a study with cathepsin-L-deficient mice.
Am J Pathol 161(6) 2047-52(2002) 12466121

Kanamori H, Takemura G, Goto K, Maruyama R, Ono K, Nagao K, Tsujimoto A, Ogino A, Takeyama T, Kawaguchi T, Watanabe T, Kawasaki M, Fujiwara T, Fujiwara H, Seishima M, Minatoguchi S.
Autophagy limits acute myocardial infarction induced by permanent coronary artery occlusion.
Am J Physiol Heart Circ Physiol 300(6) H2261-71(2011) 21421825

Kashiwagi A, Hosokawa S, Maeyama Y, Ueki R, Kaneki M, Martyn JA, Yasuhara S.
Anesthesia with Disuse Leads to Autophagy Up-regulation in the Skeletal Muscle.
Anesthesiology 122(5) 1075-83(2015) 25501690

Lo S, Yuan SS, Hsu C, Cheng YJ, Chang YF, Hsueh HW, Lee PH, Hsieh YC.
Lc3 over-expression improves survival and attenuates lung injury through increasing autophagosomal clearance in septic mice.
Ann Surg 257(2) 352-63(2013) 22968077

Inoue J, Nishiumi S, Fujishima Y, Masuda A, Shiomi H, Yamamoto K, Nishida M, Azuma T, Yoshida M.
Autophagy in the intestinal epithelium regulates Citrobacter rodentium infection.
Arch Biochem Biophys 521(1-2) 95-101(2012) 22475450

Fujishima Y, Nishiumi S, Masuda A, Inoue J, Nguyen NM, Irino Y, Komatsu M, Tanaka K, Kutsumi H, Azuma T, Yoshida M.
Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-κB activation.
Arch Biochem Biophys 506(2) 223-35(2011) 21156154

Hayashi S, Sato N, Yamamoto A, Ikegame Y, Nakashima S, Ogihara T, Morishita R.
Alzheimer disease-associated peptide, amyloid beta40, inhibits vascular regeneration with induction of endothelial autophagy.
Arterioscler Thromb Vasc Biol 29(11) 1909-15(2009) 19815818

Caramés B, Kiosses WB, Akasaki Y, Brinson DC, Eap W, Koziol J, Lotz MK.
Glucosamine activates autophagy in vitro and in vivo.
Arthritis Rheum 65(7) 1843-52(2013) 23606170

Caramés B, Olmer M, Kiosses WB, Lotz MK.
The relationship of autophagy defects to cartilage damage during joint aging in a mouse model.
Arthritis Rheumatol 67(6) 1568-76(2015) 25708836

Michiels CF, Kurdi A, Timmermans JP, De Meyer GRY, Martinet W.
Spermidine reduces lipid accumulation and necrotic core formation in atherosclerotic plaques via induction of autophagy.
Atherosclerosis 251 319-327(2016) 27450786

Kanamori H, Takemura G, Goto K, Tsujimoto A, Mikami A, Ogino A, Watanabe T, Morishita K, Okada H, Kawasaki M, Seishima M, Minatoguchi S.
Autophagic adaptations in diabetic cardiomyopathy differ between type 1 and type 2 diabetes.
Autophagy 11(7) 1146-60(2015) 26042865

Mintern JD, Macri C, Chin WJ, Panozza SE, Segura E, Patterson NL, Zeller P, Bourges D, Bedoui S, McMillan PJ, Idris A, Nowell CJ, Brown A, Radford KJ, Johnston AP, Villadangos JA.
Differential use of autophagy by primary dendritic cells specialized in cross-presentation.
Autophagy 11(6) 906-17(2015) 25950899

Chrisam M, Pirozzi M, Castagnaro S, Blaauw B, Polishchuck R, Cecconi F, Grumati P, Bonaldo P.
Reactivation of autophagy by spermidine ameliorates the myopathic defects of collagen VI-null mice.
Autophagy 11(12) 2142-52(2015) 26565691

Martinet W, Schrijvers DM, Timmermans JP, Bult H, De Meyer GR.
Immunohistochemical analysis of macroautophagy: recommendations and limitations.
Autophagy 9(3) 386-402(2013) 23242143

Chong A, Wehrly TD, Child R, Hansen B, Hwang S, Virgin HW, Celli J.
Cytosolic clearance of replication-deficient mutants reveals Francisella tularensis interactions with the autophagic pathway.
Autophagy 8(9) 1342-56(2012) 22863802

Tian F, Morimoto N, Liu W, Ohta Y, Deguchi K, Miyazaki K, Abe K.
In vivo optical imaging of motor neuron autophagy in a mouse model of amyotrophic lateral sclerosis.
Autophagy 7(9) 985-92(2011) 21628996

Grumati P, Coletto L, Schiavinato A, Castagnaro S, Bertaggia E, Sandri M, Bonaldo P.
Physical exercise stimulates autophagy in normal skeletal muscles but is detrimental for collagen VI-deficient muscles.
Autophagy 7(12) 1415-23(2011) 22024752

Tian F, Deguchi K, Yamashita T, Ohta Y, Morimoto N, Shang J, Zhang X, Liu N, Ikeda Y, Matsuura T, Abe K.
In vivo imaging of autophagy in a mouse stroke model.
Autophagy 6(8) 1107-14(2010) 20930570

Alirezaei M, Kemball CC, Flynn CT, Wood MR, Whitton JL, Kiosses WB.
Short-term fasting induces profound neuronal autophagy.
Autophagy 6(6) 702-10(2010) 20534972

Takemura G, Kanamori H, Goto K, Maruyama R, Tsujimoto A, Fujiwara H, Seishima M, Minatoguchi S.
Autophagy maintains cardiac function in the starved adult.
Autophagy 5(7) 1034-6(2009) 19587530

Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM.
Detection of autophagy in tissue by standard immunohistochemistry: possibilities and limitations.
Autophagy 2(1) 55-7(2006) 16874065

Narita H, Tanji K, Miki Y, Mori F, Wakabayashi K.
Trehalose intake and exercise upregulate a glucose transporter, GLUT8, in the brain.
Biochem Biophys Res Commun 514(3) 672-677(2019) 31078265

Nakaoka M, Iwai-Kanai E, Katamura M, Okawa Y, Mita Y, Matoba S.
An alpha-adrenergic agonist protects hearts by inducing Akt1-mediated autophagy.
Biochem Biophys Res Commun 456(1) 250-6(2015) 25446079

Zheng DM, Bian Z, Furuya N, Oliva Trejo JA, Takeda-Ezaki M, Takahashi K, Hiraoka Y, Mineki R, Taka H, Ikeda S, Komatsu M, Fujimura T, Ueno T, Ezaki J.
A treadmill exercise reactivates the signaling of the mammalian target of rapamycin (mTor) in the skeletal muscles of starved mice.
Biochem Biophys Res Commun 456(1) 519-26(2015) 25485704

Umebayashi H, Mizokami A, Matsuda M, Harada K, Takeuchi H, Tanida I, Hirata M, Kanematsu T.
Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation.
Biochem Biophys Res Commun 432(2) 268-74(2013) 23399561

Nishiumi S, Fujishima Y, Inoue J, Masuda A, Azuma T, Yoshida M.
Autophagy in the intestinal epithelium is not involved in the pathogenesis of intestinal tumors.
Biochem Biophys Res Commun 421(4) 768-72(2012) 22546555

Thomes PG, Trambly CS, Thiele GM, Duryee MJ, Fox HS, Haorah J, Donohue TM Jr.
Proteasome activity and autophagosome content in liver are reciprocally regulated by ethanol treatment.
Biochem Biophys Res Commun 417(1) 262-7(2012) 22142844

Yoshizaki T, Kusunoki C, Kondo M, Yasuda M, Kume S, Morino K, Sekine O, Ugi S, Uzu T, Nishio Y, Kashiwagi A, Maegawa H.
Autophagy regulates inflammation in adipocytes.
Biochem Biophys Res Commun 417(1) 352-7(2012) 22155234

Kurdi A, Cleenewerck M, Vangestel C, Lyssens S, Declercq W, Timmermans JP, Stroobants S, Augustyns K, De Meyer GRY, Van Der Veken P, Martinet W.
ATG4B inhibitors with a benzotropolone core structure block autophagy and augment efficiency of chemotherapy in mice.
Biochem Pharmacol 138 150-162(2017) 28642033

Sishi BJ, Loos B, van Rooyen J, Engelbrecht AM.
Autophagy upregulation promotes survival and attenuates doxorubicin-induced cardiotoxicity.
Biochem Pharmacol 85(1) 124-34(2013) 23107818

Xu L, Kanasaki M, He J, Kitada M, Nagao K, Jinzu H, Noguchi Y, Maegawa H, Kanasaki K, Koya D.
Ketogenic essential amino acids replacement diet ameliorated hepatosteatosis with altering autophagy-associated molecules.
Biochim Biophys Acta 1832(10) 1605-12(2013) 23669346

Shiraishi H, Okamoto H, Hara H, Yoshida H.
Alternative cell death of Apaf1-deficient neural progenitor cells induced by withdrawal of EGF or insulin.
Biochim Biophys Acta 1800(3) 405-15(2010) 19914347

Dennemärker J, Lohmüller T, Müller S, Aguilar SV, Tobin DJ, Peters C, Reinheckel T.
Impaired turnover of autophagolysosomes in cathepsin L deficiency.
Biol Chem 391(8) 913-22(2010) 20536383

Iwasawa T, Shinomiya T, Ota N, Shibata N, Nakata K, Shiina I, Nagahara Y.
Novel Ridaifen-B Structure Analog Induces Apoptosis and Autophagy Depending on Pyrrolidine Side Chain.
Biol Pharm Bull 42(3) 401-410(2019) 30828072

Miki Y, Tanji K, Mori F, Tatara Y, Utsumi J, Sasaki H, Kakita A, Takahashi H, Fimia GM, Wakabayashi K.
AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.
Brain Pathol 28(1) 28-42(2018) 27875637

Tambe Y, Yamamoto A, Isono T, Chano T, Fukuda M, Inoue H.
The drs tumor suppressor is involved in the maturation process of autophagy induced by low serum.
Cancer Lett 283(1) 74-83(2009) 19368996

Kawaguchi T, Takemura G, Kanamori H, Takeyama T, Watanabe T, Morishita K, Ogino A, Tsujimoto A, Goto K, Maruyama R, Kawasaki M, Mikami A, Fujiwara T, Fujiwara H, Minatoguchi S.
Prior starvation mitigates acute doxorubicin cardiotoxicity through restoration of autophagy in affected cardiomyocytes.
Cardiovasc Res 96(3) 456-65(2012) 22952253

Mizushima N, Yoshimori T, Levine B.
Methods in mammalian autophagy research.
Cell 140(3) 313-26(2010) 20144757

Fujimoto C, Iwasaki S, Urata S, Morishita H, Sakamaki Y, Fujioka M, Kondo K, Mizushima N, Yamasoba T.
Autophagy is essential for hearing in mice.
Cell Death Dis 8(5) e2780(2017) 28492547

Girault V, Gilard V, Marguet F, Lesueur C, Hauchecorne M, Ramdani Y, Laquerrière A, Marret S, Jégou S, Gonzalez BJ, Brasse-Lagnel C, Bekri S.
Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvessels.
Cell Death Dis 8(2) e2610(2017) 28182007

Wärri A, Cook KL, Hu R, Jin L, Zwart A, Soto-Pantoja DR, Liu J, Finkel T, Clarke R.
Autophagy and unfolded protein response (UPR) regulate mammary gland involution by restraining apoptosis-driven irreversible changes.
Cell Death Discov 4 40(2018) 30345078

Klinman E, Holzbaur EL.
Stress-Induced CDK5 Activation Disrupts Axonal Transport via Lis1/Ndel1/Dynein.
Cell Rep 12(3) 462-73(2015) 26166569

Ma B, Cao W, Li W, Gao C, Qi Z, Zhao Y, Du J, Xue H, Peng J, Wen J, Chen H, Ning Y, Huang L, Zhang H, Gao X, Yu L, Chen YG.
Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation.
Cell Res 24(8) 912-24(2014) 24980960

Terada M, Nobori K, Munehisa Y, Kakizaki M, Ohba T, Takahashi Y, Koyama T, Terata Y, Ishida M, Iino K, Kosaka T, Watanabe H, Hasegawa H, Ito H.
Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart.
Circ J 74(1) 203-6(2010) 19966504

Zhai P, Sciarretta S, Galeotti J, Volpe M, Sadoshima J.
Differential roles of GSK-3β during myocardial ischemia and ischemia/reperfusion.
Circ Res 109(5) 502-11(2011) 21737790

Matsui Y, Takagi H, Qu X, Abdellatif M, Sakoda H, Asano T, Levine B, Sadoshima J.
Distinct roles of autophagy in the heart during ischemia and reperfusion: roles of AMP-activated protein kinase and Beclin 1 in mediating autophagy.
Circ Res 100(6) 914-22(2007) 17332429

Maday S, Holzbaur EL.
Autophagosome biogenesis in primary neurons follows an ordered and spatially regulated pathway.
Dev Cell 30(1) 71-85(2014) 25026034

Kitada M, Ogura Y, Suzuki T, Sen S, Lee SM, Kanasaki K, Kume S, Koya D.
A very-low-protein diet ameliorates advanced diabetic nephropathy through autophagy induction by suppression of the mTORC1 pathway in Wistar fatty rats, an animal model of type 2 diabetes and obesity.
Diabetologia 59(6) 1307-17(2016) 27020449

Sauvat A, Chen G, Müller K, Tong M, Aprahamian F, Durand S, Cerrato G, Bezu L, Leduc M, Franz J, Rockenfeller P, Sadoshima J, Madeo F, Kepp O, Kroemer G.
Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids.
EBioMedicine 30 261-272(2018) 29606629

Tang AH, Rando TA.
Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation.
EMBO J 33(23) 2782-97(2014) 25316028

Napolitano G, Johnson JL, He J, Rocca CJ, Monfregola J, Pestonjamasp K, Cherqui S, Catz SD.
Impairment of chaperone-mediated autophagy leads to selective lysosomal degradation defects in the lysosomal storage disease cystinosis.
EMBO Mol Med 7(2) 158-74(2015) 25586965

Lee JE, Oh HA, Song H, Jun JH, Roh CR, Xie H, Dey SK, Lim HJ.
Autophagy regulates embryonic survival during delayed implantation.
Endocrinology 152(5) 2067-75(2011) 21363932

Segi N, Ozaki T, Suzuki Y, Ouchida J, Imagama S, Kadomatsu K, Sakamoto K.
Close association of polarization and LC3, a marker of autophagy, in axon determination in mouse hippocampal neurons.
Exp Neurol 354 114112(2022) 35568188

Chang AL, Ulrich A, Suliman HB, Piantadosi CA.
Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis.
Free Radic Biol Med 78 179-89(2015) 25450328

Mukherjee R, Mareninova OA, Odinokova IV, Huang W, Murphy J, Chvanov M, Javed MA, Wen L, Booth DM, Cane MC, Awais M, Gavillet B, Pruss RM, Schaller S, Molkentin JD, Tepikin AV, Petersen OH, Pandol SJ, Gukovsky I, Criddle DN, Gukovskaya AS, Sutton R, NIHR Pancreas Biomedical Research Unit.
Mechanism of mitochondrial permeability transition pore induction and damage in the pancreas: inhibition prevents acute pancreatitis by protecting production of ATP.
Gut 65(8) 1333-46(2016) 26071131

Ni HM, Bockus A, Boggess N, Jaeschke H, Ding WX.
Activation of autophagy protects against acetaminophen-induced hepatotoxicity.
Hepatology 55(1) 222-32(2012) 21932416

French CJ, Taatjes DJ, Sobel BE.
Autophagy in myocardium of murine hearts subjected to ischemia followed by reperfusion.
Histochem Cell Biol 134(5) 519-26(2010) 20931339

Mariño G, Ugalde AP, Salvador-Montoliu N, Varela I, Quirós PM, Cadiñanos J, van der Pluijm I, Freije JM, López-Otín C.
Premature aging in mice activates a systemic metabolic response involving autophagy induction.
Hum Mol Genet 17(14) 2196-211(2008) 18443001

Kim SJ, Hong EH, Lee BR, Park MH, Kim JW, Pyun AR, Kim YJ, Chang SY, Chin YW, Ko HJ.
α-Mangostin Reduced ER Stress-mediated Tumor Growth through Autophagy Activation.
Immune Netw 12(6) 253-60(2012) 23396851

Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS, Gonzalez-Nuñez M, Austin-Williams SN, Cooper D, Vázquez-Martínez L, Fu T, De Rossi G, Golding M, Voisin MB, Boulanger CM, Kubota Y, Muller WA, Tooze SA, Nightingale TD, Collinson L, Perretti M, Aksoy E, Nourshargh S.
Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation.
Immunity 54(9) 1989-2004.e9(2021) 34363750

Sciarretta S, Yee D, Nagarajan N, Bianchi F, Saito T, Valenti V, Tong M, Del Re DP, Vecchione C, Schirone L, Forte M, Rubattu S, Shirakabe A, Boppana VS, Volpe M, Frati G, Zhai P, Sadoshima J.
Trehalose-Induced Activation of Autophagy Improves Cardiac Remodeling After Myocardial Infarction.
J Am Coll Cardiol 71(18) 1999-2010(2018) 29724354

Kim SI, Na HJ, Ding Y, Wang Z, Lee SJ, Choi ME.
Autophagy promotes intracellular degradation of type I collagen induced by transforming growth factor (TGF)-β1.
J Biol Chem 287(15) 11677-88(2012) 22351764

Yoshida A, Kanamori H, Naruse G, Minatoguchi S, Iwasa M, Yamada Y, Mikami A, Kawasaki M, Nishigaki K, Minatoguchi S.
(Pro)renin Receptor Blockade Ameliorates Heart Failure Caused by Chronic Kidney Disease.
J Card Fail 25(4) 286-300(2019) 30769036

Terawaki S, Camosseto V, Prete F, Wenger T, Papadopoulos A, Rondeau C, Combes A, Rodriguez Rodrigues C, Vu Manh TP, Fallet M, English L, Santamaria R, Soares AR, Weil T, Hammad H, Desjardins M, Gorvel JP, Santos MA, Gatti E, Pierre P.
RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4.
J Cell Biol 210(7) 1133-52(2015) 26416964

Maday S, Wallace KE, Holzbaur EL.
Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons.
J Cell Biol 196(4) 407-17(2012) 22331844

Lam HC, Cloonan SM, Bhashyam AR, Haspel JA, Singh A, Sathirapongsasuti JF, Cervo M, Yao H, Chung AL, Mizumura K, An CH, Shan B, Franks JM, Haley KJ, Owen CA, Tesfaigzi Y, Washko GR, Quackenbush J, Silverman EK, Rahman I, Kim HP, Mahmood A, Biswal SS, Ryter SW, Choi AM.
Histone deacetylase 6-mediated selective autophagy regulates COPD-associated cilia dysfunction.
J Clin Invest 123(12) 5212-30(2013) 24200693

Mareninova OA, Hermann K, French SW, O'Konski MS, Pandol SJ, Webster P, Erickson AH, Katunuma N, Gorelick FS, Gukovsky I, Gukovskaya AS.
Impaired autophagic flux mediates acinar cell vacuole formation and trypsinogen activation in rodent models of acute pancreatitis.
J Clin Invest 119(11) 3340-55(2009) 19805911

Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM.
In situ detection of starvation-induced autophagy.
J Histochem Cytochem 54(1) 85-96(2006) 16148314

Hoshino A, Matoba S, Iwai-Kanai E, Nakamura H, Kimata M, Nakaoka M, Katamura M, Okawa Y, Ariyoshi M, Mita Y, Ikeda K, Ueyama T, Okigaki M, Matsubara H.
p53-TIGAR axis attenuates mitophagy to exacerbate cardiac damage after ischemia.
J Mol Cell Cardiol 52(1) 175-84(2012) 22044588

Maday S, Holzbaur EL.
Compartment-Specific Regulation of Autophagy in Primary Neurons.
J Neurosci 36(22) 5933-45(2016) 27251616

Wong YC, Holzbaur EL.
The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation.
J Neurosci 34(4) 1293-305(2014) 24453320

Song JW, Misgeld T, Kang H, Knecht S, Lu J, Cao Y, Cotman SL, Bishop DL, Lichtman JW.
Lysosomal activity associated with developmental axon pruning.
J Neurosci 28(36) 8993-9001(2008) 18768693

Hylin MJ, Zhao J, Tangavelou K, Rozas NS, Hood KN, MacGowan JS, Moore AN, Dash PK.
A role for autophagy in long-term spatial memory formation in male rodents.
J Neurosci Res 96(3) 416-426(2018) 29230855

Kemball CC, Alirezaei M, Flynn CT, Wood MR, Harkins S, Kiosses WB, Whitton JL.
Coxsackievirus infection induces autophagy-like vesicles and megaphagosomes in pancreatic acinar cells in vivo.
J Virol 84(23) 12110-24(2010) 20861268

Zhang Y, Nakano D, Guan Y, Hitomi H, Uemura A, Masaki T, Kobara H, Sugaya T, Nishiyama A.
A sodium-glucose cotransporter 2 inhibitor attenuates renal capillary injury and fibrosis by a vascular endothelial growth factor-dependent pathway after renal injury in mice.
Kidney Int 94(3) 524-535(2018) 30045814

Gubbiotti MA, Neill T, Frey H, Schaefer L, Iozzo RV.
Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy.
Matrix Biol 48 14-25(2015) 26344480

Frost LS, Dhingra A, Reyes-Reveles J, Boesze-Battaglia K.
The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo.
Methods Enzymol 587 43-54(2016) 28253971

Dong A, Kulkarni VV, Maday S.
Methods for Imaging Autophagosome Dynamics in Primary Neurons.
Methods Mol Biol 1880 243-256(2019) 30610702

Mizushima N, Yamamoto A, Matsui M, Yoshimori T, Ohsumi Y.
In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker.
Mol Biol Cell 15(3) 1101-11(2004) 14699058

Hoshino A, Mita Y, Okawa Y, Ariyoshi M, Iwai-Kanai E, Ueyama T, Ikeda K, Ogata T, Matoba S.
Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart.
Nat Commun 4 2308(2013) 23917356

Pengo N, Scolari M, Oliva L, Milan E, Mainoldi F, Raimondi A, Fagioli C, Merlini A, Mariani E, Pasqualetto E, Orfanelli U, Ponzoni M, Sitia R, Casola S, Cenci S.
Plasma cells require autophagy for sustainable immunoglobulin production.
Nat Immunol 14(3) 298-305(2013) 23354484

Maejima Y, Kyoi S, Zhai P, Liu T, Li H, Ivessa A, Sciarretta S, Del Re DP, Zablocki DK, Hsu CP, Lim DS, Isobe M, Sadoshima J.
Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2.
Nat Med 19(11) 1478-88(2013) 24141421

Nakai A, Yamaguchi O, Takeda T, Higuchi Y, Hikoso S, Taniike M, Omiya S, Mizote I, Matsumura Y, Asahi M, Nishida K, Hori M, Mizushima N, Otsu K.
The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress.
Nat Med 13(5) 619-24(2007) 17450150

Adolph TE, Tomczak MF, Niederreiter L, Ko HJ, Böck J, Martinez-Naves E, Glickman JN, Tschurtschenthaler M, Hartwig J, Hosomi S, Flak MB, Cusick JL, Kohno K, Iwawaki T, Billmann-Born S, Raine T, Bharti R, Lucius R, Kweon MN, Marciniak SJ, Choi A, Hagen SJ, Schreiber S, Rosenstiel P, Kaser A, Blumberg RS.
Paneth cells as a site of origin for intestinal inflammation.
Nature 503(7475) 272-6(2013) 24089213

Fimia GM, Stoykova A, Romagnoli A, Giunta L, Di Bartolomeo S, Nardacci R, Corazzari M, Fuoco C, Ucar A, Schwartz P, Gruss P, Piacentini M, Chowdhury K, Cecconi F.
Ambra1 regulates autophagy and development of the nervous system.
Nature 447(7148) 1121-5(2007) 17589504

Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N.
The role of autophagy during the early neonatal starvation period.
Nature 432(7020) 1032-6(2004) 15525940

Andrea K. H. Stavoe, Erika L. F. Holzbaur
Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons
Neuromethods 23-40(2022)

Goldsmith J, Ordureau A, Harper JW, Holzbaur ELF.
Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.
Neuron 110(6) 967-976.e8(2022) 35051374

Matsuda S, Miura E, Matsuda K, Kakegawa W, Kohda K, Watanabe M, Yuzaki M.
Accumulation of AMPA receptors in autophagosomes in neuronal axons lacking adaptor protein AP-4.
Neuron 57(5) 730-45(2008) 18341993

Tominaga T, Goto M, Onoue T, Mizoguchi A, Sugiyama M, Tsunekawa T, Hagiwara D, Morishita Y, Ito Y, Iwama S, Suga H, Banno R, Arima H.
Sequestosome 1 (SQSTM1/p62) maintains protein folding capacity under endoplasmic reticulum stress in mouse hypothalamic organotypic culture.
Neurosci Lett 656 103-107(2017) 28619261

Meckes JK, Caramés B, Olmer M, Kiosses WB, Grogan SP, Lotz MK, D'Lima DD.
Compromised autophagy precedes meniscus degeneration and cartilage damage in mice.
Osteoarthritis Cartilage 25(11) 1880-1889(2017) 28801209

Kwok J, Onuma H, Olmer M, Lotz MK, Grogan SP, D'Lima DD.
Histopathological analyses of murine menisci: implications for joint aging and osteoarthritis.
Osteoarthritis Cartilage 24(4) 709-18(2016) 26585241

Hariharan N, Ikeda Y, Hong C, Alcendor RR, Usui S, Gao S, Maejima Y, Sadoshima J.
Autophagy plays an essential role in mediating regression of hypertrophy during unloading of the heart.
PLoS One 8(1) e51632(2013) 23308102

Patel AS, Lin L, Geyer A, Haspel JA, An CH, Cao J, Rosas IO, Morse D.
Autophagy in idiopathic pulmonary fibrosis.
PLoS One 7(7) e41394(2012) 22815997

Cao L, Kobayakawa S, Yoshiki A, Abe K.
High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
PLoS One 7(3) e33876(2012) 22479464

Chinzei R, Masuda A, Nishiumi S, Nishida M, Onoyama M, Sanuki T, Fujita T, Moritoh S, Itoh T, Kutsumi H, Mizuno S, Azuma T, Yoshida M.
Vitamin K3 attenuates cerulein-induced acute pancreatitis through inhibition of the autophagic pathway.
Pancreas 40(1) 84-94(2011) 20881898

Brosius Lutz A, Chung WS, Sloan SA, Carson GA, Zhou L, Lovelett E, Posada S, Zuchero JB, Barres BA.
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury.
Proc Natl Acad Sci U S A 114(38) E8072-E8080(2017) 28874532

Luo SM, Ge ZJ, Wang ZW, Jiang ZZ, Wang ZB, Ouyang YC, Hou Y, Schatten H, Sun QY.
Unique insights into maternal mitochondrial inheritance in mice.
Proc Natl Acad Sci U S A 110(32) 13038-43(2013) 23878233

Tsvetkov AS, Miller J, Arrasate M, Wong JS, Pleiss MA, Finkbeiner S.
A small-molecule scaffold induces autophagy in primary neurons and protects against toxicity in a Huntington disease model.
Proc Natl Acad Sci U S A 107(39) 16982-7(2010) 20833817

Bang S, Shin H, Song H, Suh CS, Lim HJ.
Autophagic activation in vitrified-warmed mouse oocytes.
Reproduction 148(1) 11-9(2014) 24760879

Anuradha Dhingra, Desiree Alexander, Juan Reyes-Reveles, Rachel Sharp, and Kathleen Boesze-Battaglia.
Chapter 74 Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.
Retinal Degenerative Diseases: Mechanisms and Experimental Therapy. John D. Ash, ‎Robert E. Anderson, ‎Matthew M. LaVail, Catherine Bowes Rickman. ()

Miyake T, Sakai N, Tamai A, Sato K, Kamikawa Y, Miyagawa T, Ogura H, Yamamura Y, Oshima M, Nakagawa S, Sagara A, Shinozaki Y, Toyama T, Kitajima S, Hara A, Iwata Y, Shimizu M, Furuichi K, Kaneko S, Wada T.
Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells.
Sci Rep 10(1) 14292(2020) 32868830

Denaës T, Lodder J, Chobert MN, Ruiz I, Pawlotsky JM, Lotersztajn S, Teixeira-Clerc F.
The Cannabinoid Receptor 2 Protects Against Alcoholic Liver Disease Via a Macrophage Autophagy-Dependent Pathway.
Sci Rep 6 28806(2016) 27346657

Choy A, Dancourt J, Mugo B, O'Connor TJ, Isberg RR, Melia TJ, Roy CR.
The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation.
Science 338(6110) 1072-6(2012) 23112293

Lee HK, Lund JM, Ramanathan B, Mizushima N, Iwasaki A.
Autophagy-dependent viral recognition by plasmacytoid dendritic cells.
Science 315(5817) 1398-401(2007) 17272685

Nakagawa I, Amano A, Mizushima N, Yamamoto A, Yamaguchi H, Kamimoto T, Nara A, Funao J, Nakata M, Tsuda K, Hamada S, Yoshimori T.
Autophagy defends cells against invading group A Streptococcus.
Science 306(5698) 1037-40(2004) 15528445

Sishi BJ, Loos B, van Rooyen J, Engelbrecht AM.
Doxorubicin induces protein ubiquitination and inhibits proteasome activity during cardiotoxicity.
Toxicology 309 23-9(2013) 23639627

Tanemura M, Saga A, Kawamoto K, Machida T, Deguchi T, Nishida T, Sawa Y, Doki Y, Mori M, Ito T.
Rapamycin induces autophagy in islets: relevance in islet transplantation.
Transplant Proc 41(1) 334-8(2009) 19249550