Strain Data Sheet

RBRC00805

Strain Information

Image
BRC No.RBRC00805
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Hoxc<tm1Nogu>/NoguRbrc
Former Common nameHox C cluster Knockout mice
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hirofumi Suemori, Meiji Institute of Health Science in 1996.
Strain descriptionB6;129S-Hoxc<tm1Nogu>. Hoxc gene deficient mice. Homozygous mutant mice are embryonic lethal.
Colony maintenancebackcross to C57BL/6N (Heterozygote x C57BL/6NJcl)
References
Hox C cluster genes are dispensable for overall body plan of mouse embryonic development.
Suemori H, Noguchi S
Dev Biol., 220, 333-342 (2000). 10753520

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hoxchomeobox C cluster15Hoxctargeted mutation 1, Shigeru Noguchi
loxPphage P1 loxP15loxP
puropuromycin acyltransferase (Streptomyces alboniger)15puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal caudal vertebrae morphology(MP:0002759)

  • abnormal lung development(MP:0001176)

  • abnormal neuromuscular synapse morphology(MP:0001053)

  • abnormal pulmonary alveolus morphology(MP:0002270)

  • abnormal rib morphology(MP:0000150)
    • more 8 phenotypes
  • abnormal thoracic vertebrae morphology(MP:0003047)

  • abnormal vertebrae morphology(MP:0000137)

  • absent vibrissae(MP:0001284)

  • caudal vertebral transformation(MP:0004614)

  • lumbar vertebral transformation(MP:0004616)

  • neonatal lethality, complete penetrance(MP:0011087)

  • sacral vertebral transformation(MP:0004617)

  • thoracic vertebral transformation(MP:0004618)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse homeo box C cluster genomic DNA, PEF1a (elongation factor 1a promoter), Phage P1 loxP, Streptomyces alboniger puromycin-resistance gene
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Del. Biol., 220, 333-342 (2000).
    DepositorHirofumi Suemori (Kanagawa Academy of Science and Technology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Schorderet P, Duboule D.
    Structural and functional differences in the long non-coding RNA hotair in mouse and human.
    PLoS Genet 7(5) e1002071(2011) 21637793
    Tschopp P, Fraudeau N, Béna F, Duboule D.
    Reshuffling genomic landscapes to study the regulatory evolution of Hox gene clusters.
    Proc Natl Acad Sci U S A 108(26) 10632-7(2011) 21670281