RBRC00805

Information1

Image
BRC No.RBRC00805
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Hoxc<tm1Nogu>/NoguRbrc
Former Common nameHox C cluster Knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hirofumi Suemori, Meiji Institute of Health Science in 1996.
Strain descriptionB6;129S-Hoxc<tm1Nogu>. Hoxc gene deficient mice. Homozygous mutant mice are embryonic lethal.
Colony maintenancebackcross to C57BL/6N (Heterozygote x C57BL/6NJcl)
ReferencesDev Biol., 220, 333-342 (2000). 10753520

Health Report

Examination Date / Room / Rack2009/10/19Room:3-CRack:B
2009/08/24Room:3-CRack:B
2009/06/22Room:3-CRack:B
2009/04/21Room:3-CRack:B
2009/04/20Room:3-CRack:B
2009/02/23Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hoxchomeo box C cluster15Hoxc<tm1Nogu>targeted mutation 1, Shigeru Noguchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)15puro

Information2

Donor DNAmouse homeo box C cluster genomic DNA, PEF1a (elongation factor 1a promoter), Phage P1 loxP, Streptomyces alboniger puromycin-resistance gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Del. Biol., 220, 333-342 (2000).
DepositorHirofumi Suemori (Kanagawa Academy of Science and Technology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Schorderet P, Duboule D.
Structural and functional differences in the long non-coding RNA hotair in mouse and human.
PLoS Genet. 7(5) e1002071(2011) 21637793

Tschopp P, Fraudeau N, Bena F, Duboule D.
Reshuffling genomic landscapes to study the regulatory evolution of Hox gene clusters.
Proc. Natl. Acad. Sci. U.S.A. 108(26) 10632-7(2011) 21670281