Strain Data Sheet

RBRC00804

Strain Information

Image
BRC No.RBRC00804
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-S100b<tm1Ito>/ItoRbrc
Former Common nameS100b-KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped Hiroshi Nishiyama and Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6.
Strain descriptionS100b gene knockout mice. Exon 2 and part of exon 3 of S100b gene were replaced with a floxed neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceSibling Mating
References
Glial protein S100B modulates long-term neuronal synaptic plasticity.
Nishiyama H, Knopfel T, Endo S, Itohara S
Proc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002). 11891290

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
S100b
MGI:98217		S100 protein, beta polypeptide, neural10S100b<tm1Ito>
MGI:2451049		targeted mutation 1, Shigeyoshi Itohara
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin, mouse calcium-binding protein S100b genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Liu J, Wang H, Zhang L, Xu Y, Deng W, Zhu H, Qin C.
    S100B transgenic mice develop features of Parkinson's disease.
    Arch Med Res 42(1) 1-7(2011) 21376255