Strain Data Sheet

RBRC00804

Information1

Image
BRC No.RBRC00804
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-S100b<tm1Ito>/ItoRbrc
Former Common nameS100b-KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped Hiroshi Nishiyama and Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6.
Strain descriptionS100b gene knockout mice. Exon 2 and part of exon 3 of S100b gene were replaced with a floxed neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceSibling Mating
ReferencesProc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002). 11891290

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
S100bcalcium-binding protein S100b10S100b<tm1Ito>targeted mutation 1, Shigeyoshi ItoharaBpb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin, mouse calcium-binding protein S100b genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Liu J, Wang H, Zhang L, Xu Y, Deng W, Zhu H, Qin C.
S100B transgenic mice develop features of Parkinson's disease.
Arch Med Res 42(1) 1-7(2011) 21376255