RBRC00782

Information1

Image
BRC No.RBRC00782
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gipr<tm1Yse>/Rbrc
Former Common nameGIPR Knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Yutaka Seino, Kyoto University in 1999. A neomycin cassette was inserted to replace exons 4 and 5 of GIPR gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6.129P2-Gipr<tm1Yse>. Gastric inhibitory polypeptide (GIP) is a gastrointestinal peptide hormone synthesized by K cells in the duodenum and small intestine, GIP plays a role in gastric secretory inhibition, gastric mobility, and insulin release by binding to its specific receptor, the GIP receptor (GIPR). Homozygous mutant mice for Gipr<tm1Yse> targeted mutation are fertile and viable, but exhibit hyperglycemia after high-fat diet ingestion. This strain is useful for elucidating of the signaling mechanism of GIP from pancreatic beta cell to intestine.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)Homozygous mice are fertile and viable.
ReferencesProc. Natl. Acad. Sci. USA, 96, 14843-14847 (1999). 10611300

Health Report

Examination Date / Room / Rack2011/04/18Room:3-CRack:J
2011/02/21Room:3-CRack:J
2010/12/13Room:3-CRack:J
2010/10/18Room:3-CRack:J
2010/08/23Room:3-CRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Giprgastric inhibitory polypeptide receptor7Gipr<tm1Yse>targeted mutation 1, Yutaka Seino

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse GIP receptor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Miyawaki K, et al. Proc Natl Acad Sci USA, 96, 14843-14847 (1999).
DepositorYutaka Seino (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Iida A, Seino Y, Fukami A, Maekawa R, Yabe D, Shimizu S, Kinoshita K, Takagi Y, Izumoto T, Ogata H, Ishikawa K, Ozaki N, Tsunekawa S, Hamada Y, Oiso Y, Arima H, Hayashi Y.
Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin.
Diabetologia (2016) 27053237