|Former Common name||Oct-GFP#11, TgN(GOFGFP)11Imeg|
|ES Cell line|
|Strain development||Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 2001. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.|
|Strain description||Oct-4/EGFP transgenic mice carry EGFP (enhanced green fluorescence protein) cDNA under control of an Oct4 18-kb genomic fragment containing the minimal promoter and proximal and distal enhanced. (Dev Biol 2003). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.|
|Colony maintenance||Carrier x Carrier [or Crossing to C57BL/6JJcl]|
|References||Dev. Biol., 258, 209-225 (2003). 12781694|
|Examination Date / Room / Rack|
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNOct 3/4 promoter
|Donor DNA||Oct 3/4 promoter, Jellyfish GFP cDNA, SV40 polyA|
|Research application||Cell Biology Research|
Developmental Biology Research
Fluorescent Proteins/lacZ System
|Specific Term and Conditions||The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. |
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
|Depositor||Kuniya Abe (RIKEN)|
|Strain Status||Frozen embryos|
|Strain Availability||Recovered litters from cryopreserved embryos (2 to 4 months)|
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
|Additional Info.||Necessary documents for ordering:|
Genotyping protocol -PCR-
BRC mice in Publications
Li Y, Zhang Q, Yin X, Yang W, Du Y, Hou P, Ge J, Liu C, Zhang W, Zhang X, Wu Y, Li H, Liu K, Wu C, Song Z, Zhao Y, Shi Y, Deng H.
Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules.
Cell Res 21(1) 196-204(2011) 20956998
Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T.
Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system.
Dev Biol 346(1) 90-101(2010) 20659443
Qing T, Liu H, Wei W, Ye X, Shen W, Zhang D, Song Z, Yang W, Ding M, Deng H.
Mature oocytes derived from purified mouse fetal germ cells.
Hum Reprod 23(1) 54-61(2008) 18000170
Mizuno N, Kosaka M.
Novel variants of Oct-3/4 gene expressed in mouse somatic cells.
J Biol Chem 283(45) 30997-1004(2008) 18765667
Yang W, Wei W, Shi C, Zhu J, Ying W, Shen Y, Ye X, Fang L, Duo S, Che J, Shen H, Ding S, Deng H.
Pluripotin combined with leukemia inhibitory factor greatly promotes the derivation of embryonic stem cell lines from refractory strains.
Stem Cells 27(2) 383-9(2009) 19056907