Strain Data Sheet

RBRC00762

Information1

Image
BRC No.RBRC00762
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC;B6-a/a-Tyrp1<b>-Kit<W-sh>/Rbrc
Former Common nameW Sash Brown
H-2 Haplotype
ES Cell line
Background strain
Appearancewhite coat with black eyes [a/a b/b C/C sh/sh]
Strain developmentC57BL/6-Kit<W-sh> mice were deposited by Dr. Yukihiko Kitamuta, Osaka University to the RIKEN BRC. The mice were backcrossed to BALB/c for several generation, then maintained by sister-brother mating as homozygous state.
Strain descriptionThe Kit<W-sh> mutation arose spontaneously in the (C3H/HeN x 101/H)F1 at the MRC (Lyon et al., 1982). Homozygous mice are white coat with black eyes. Heterozygotes are black with a white sash. This strain is useful for study of melanogenesis and neural crest migration.
Colony maintenanceSibling mating (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
ReferencesBlood, 15, 1448-1453 (1992) 1381627
Blood, 83, 3509-3516 (1994). 7515715
Development, 118, 705-717 (1993). 7521281
Blood, 94, 2658-2666 (1999). 10515869
Am. J. Pathol., 167, 835-848 (2005). 16127161

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
anonagouti2a<a>nonagouti

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrp1tyrosinase-related protein 14Tyrp1<b>brown

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kitkit oncogene5Kit<W-Sh>sash

Information2

Donor DNA
Research applicationReproductive Biology Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Iino S, Horiguchi S, Horiguchi K, Hashimoto T.
Interstitial cells of Cajal in Wsh/Wsh c-kit mutant mice.
J Smooth Muscle Res 56(0) 58-68(2020) 33132281

Iino S, Horiguchi K, Nojyo Y.
W(sh)/W(sh) c-Kit mutant mice possess interstitial cells of Cajal in the deep muscular plexus layer of the small intestine.
Neurosci Lett 459(3) 123-6(2009) 19427361