RBRC00757

Information1

Image
BRC No.RBRC00757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Ptpn7<tm1Bwz>/Rbrc
Former Common nameHePTP Knockout mice
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentB6;129P2-Ptpn7<tm1Bwz>. Hematopoietic protein tyrosine phosphatase (HePTP) is a hematopoiesis-specific cytoplasmic protein tyrosine phosphatase that is induced in primary lymphocytes by extracellular stimuli. This knockout mouse strain is useful for investigating the function of the HePTP gene.
Strain descriptionDeveloped by Dr. Haruhiko Suzuki, University of Toronto in 1994. A neomycin selection cassette was inserted into the second exon of the HePTP gene. The HePTP deficient mice were backcrossed to C57BL/6 for 4 times.
Colony maintenanceHomozygous mutant mice were viable and fertile.
ReferencesMol. Cell. Biol., 21, 6851-6858 (2001). 11564869

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptpn7protein tyrosine phosphatase, non-receptor type 71Ptpn7<tm1Bwz>targeted mutation 1, Brent W Zanke

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Ptpn7 genomic DNA
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorHaruhiko Suzuki (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Contact Information

BRC mice in Publications

No Data