RBRC00702

Information1

Image
BRC No.RBRC00702
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-TgH(Zic1)
Former Common name
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Aruga J (Aruga J et al. J Neurosci 18, 284-293, 1998).The mice were frozen-stored at 2-cell stage after in vitro fertilization between a heterozygous male and C57BL/6J females. Live mice were recovered and born on April 28, 2003.Deposited to RIKEN BioResource Center, RIKEN Tsukuba Institute in 2002.
Strain descriptionZic genes encode zinc finger proteins. These genes are homologs of the Drosophila pair-rule gene odd-paired. This strain was developed to clarify the function of Zic1 gene. Homozygous Zic1 deficient mice show remarkable ataxia during postnatal development, and die within 1 month. Their cerebella are hypoplastic and missing a lobule in the anterior lobe. A bromodeoxyuridine labeling study indicated a reduction both in the proliferating cell fraction in the external germinal layer (EGL), from embryonic day14, and in forward movement of the EGL (Aruga J et al. J Neurosci 18, 284-293, 1998).
Colony maintenanceMice were maintained by successive backcrossing heterozygotes to C57BL/6 mice.
ReferencesJ. Neurosci., 18, 284-293 (1998). 9412507

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zic1zinc finger protein of the cerebellum 19Zic1<tm1Jaru>targeted mutation 1, Jun Aruga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse zinc finger protein of the cerebellum 1(nervous system specific transcription factor) Zic1 genomic DNA, mouse phosphoglycerate kinase promoter, E. coli neo
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 18, 284-293 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorJun Aruga (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP

BRC mice in Publications

Inessa GRINBERG, Hope NORTHRUP, Holly ARDINGER, Chitra PRASAD, William B DOBYNS, Kathleen J MILLEN
Heterozygous deletion of the linked genes ZIC1 and ZIC4 is involved in Dandy-Walker malformation.
Nat. Genet. 10 1053-1055(2004) 15338008