RBRC00694

Information1

Image
BRC No.RBRC00694
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(KRT14-Kitl)1Takk/TakkRbrc
Former Common namehk14-SCF (human cytokeratin14 promoter + mouse stem cell factor cDNA) B6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack and prominent epidermal pigmentation [a/a B/B C/C Tg/+]
Strain developmentDeveloped by Dr. Takahiro Kunisada, Tottori University at 1996. The murine cDNA fragment under the human cytokeration 14 promoter was injected into the pronuclei of fertilized egges. The transgenic mice were maintained by brother-sister mating after backcrossing to C57BL/6Cr mice for 10 generations.Generation:N10F20+F1F7
Strain descriptionThese transgenic mice develop prominent epidermal pigmentation by overexpression of the mouse SCF gene controlled by the human keratin 14 promoter. The local high concentrations of soluble SCF have been implicated in the pathogenesis of human cutaneous mastocytosis. The phenotypes of these transgenic mice conform with that of human mastocytosis. This strain is more useful for studying of cutaneous mastocytosis and epidermal melanocytes biology than using of normal skin mice.
Colony maintenanceSibling Mating (Hemizygote x Hemizygote, Wild-type)Reproductive performance is good.
ReferencesDevelopment, 125, 2915-2923 (1998). 9655813
J. Exp. Med., 187, 1565-1573 (1998). 9584135

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Kitlkit ligandUNKitlhuman cytokeratin14 promoter, poly A

Information2

Donor DNAhuman cytokeratin14 promoter, rabbit beta globin intron, mouse stem cell factor cDNA, human cytokeratin14 poly A signal
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorTakahiro Kunisada (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data