Strain Data Sheet

RBRC00694

Strain Information

Image
BRC No.RBRC00694
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(KRT14-Kitl)1Takk/TakkRbrc
Former Common namehk14-SCF (human cytokeratin14 promoter + mouse stem cell factor cDNA) B6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack and prominent epidermal pigmentation [a/a B/B C/C Tg/+]
Strain developmentDeveloped by Dr. Takahiro Kunisada, Tottori University at 1996. The murine cDNA fragment under the human cytokeration 14 promoter was injected into the pronuclei of fertilized egges. The transgenic mice were maintained by brother-sister mating after backcrossing to C57BL/6Cr mice for 10 generations.Generation:N10F20+F1F7
Strain descriptionThese transgenic mice develop prominent epidermal pigmentation by overexpression of the mouse SCF gene controlled by the human keratin 14 promoter. The local high concentrations of soluble SCF have been implicated in the pathogenesis of human cutaneous mastocytosis. The phenotypes of these transgenic mice conform with that of human mastocytosis. This strain is more useful for studying of cutaneous mastocytosis and epidermal melanocytes biology than using of normal skin mice.
Colony maintenanceSibling Mating (Hemizygote x Hemizygote, Wild-type)Reproductive performance is good.
References
Murine cutaneous mastocytosis and epidermal melanocytosis induced by keratinocyte expression of transgenic stem cell factor.
Kunisada T, Lu S Z, Yoshida H, Nishikawa S, Nishikawa S, Mizoguchi M, Hayashi S, Tyrrell L, Williams D A, Wang X, Longley B J
J. Exp. Med., 187, 1565-1573 (1998). 9584135

Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors.
Kunisada T, Yoshida H, Yamazaki H, Miyamoto A, Hemmi H, Nishimura E, Shultz L D, Nishikawa S, Hayashi S
Development, 125, 2915-2923 (1998). 9655813

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:L
2024/05/27Room:4-BRack:L
2024/02/26Room:4-BRack:L
2023/11/27Room:4-BRack:L
2023/09/28Room:4-BRack:L
2023/08/28Room:4-BRack:L
2023/05/29Room:4-BRack:L
2023/02/27Room:4-BRack:L

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2rabbit beta globin intronUNHBB2
  • autosomal dominant nonsyndromic hearing loss 69(MedGEN)

  • hyperpigmentation with or without hypopigmentation, familial progressive(MedGEN)
    • Kitlkit ligandUNKitlhuman cytokeratin14 promoter, poly A
  • autosomal dominant nonsyndromic hearing loss 69(MedGEN)

  • hyperpigmentation with or without hypopigmentation, familial progressive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal epidermal pigmentation(MP:0009387)

  • increased mast cell number(MP:0000324)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman cytokeratin14 promoter, rabbit beta globin intron, mouse stem cell factor cDNA, human cytokeratin14 poly A signal
    Research application
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorTakahiro Kunisada (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data