RBRC00673

Information1

Image
BRC No.RBRC00673
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTAM1floxmice
Former Common nameB6.Cg-Stam<tm2Sug> Stam2<tm1Sug>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6NCrSlc
Appearanceblack
Strain developmentDeveloped by Kazuo Sugamura, Tohoku University Graduate School of Medicine. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain descriptionSTAM1 floxed, STAM2 KO double mutant mice.
Colony maintenanceStam: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
ReferencesMol Cell Biol., 22(24): 8648-8658 (2002). 12446783

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Stamsignal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stam<tm2Sug>targeted mutation 2, Kazuo Sugamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Stam2signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2<tm1Sug>targeted mutation 1, Kazuo Sugamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuo Sugamura (Miyagi Prefectural Hospital Organization)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data