RBRC00672

Information1

Image
BRC No.RBRC00672
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Stam<tm1Sug> Stam2<tm1Sug>/SugRbrc
Former Common nameC.Cg-Stam<tm1Sug> Stam2<tm1Sug>, STAM1+/-, STAM2+/-
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainBALB/c
Appearancealbino
Strain developmentDeveloped by Kazuo Sugamura, Tohoku University Graduate School of Medicine in 1999. The mutant mice were backcrossed to BALB/c over 6 times.
Strain descriptionSTAM1, STAM2 double knockout mice. STAM1 homozygous mutant mice show growth defect with aging, and neurological abnormality (reduction of pyramidal cells in CA3 of the hippocampus). STAM2 homozygous mutant mice are indistinguishable from controls. Double homozygous mutant mice are embryonic lethal.
Colony maintenanceStam: Heterozygote x Wild-type [or Crossing to BALB/cCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to BALB/cCrSlc]
ReferencesMol. Cell. Biol., 21, 3807-3819 (2001). 11340172

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Stamsignal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stam<tm1Sug>targeted mutation 1, Kazuo Sugamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Stam2signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2<tm1Sug>targeted mutation 1, Kazuo Sugamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse STAM1 and STAM2 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuo Sugamura (Miyagi Prefectural Hospital Organization)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data