Strain Data Sheet

RBRC00467

update : 2024-08-20

Strain Information
Image
BRC No.	RBRC00467
Type	Targeted Mutation
Species	Mus musculus
Strain name	STOCK Coq7<tm1Tsh>/ShsRbrc
Former Common name	Clk-1 Knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance	unfixed
Strain development	Developed by Dr. Takuji Shirasawa at Tokyo Metropolitan Institute of Gerontology in 2001. A neomycin selection cassette was inserted into the exon 2 of the Clk1 gene. 129, C57BL/6 and ICR mixed background.
Strain description	The Clk1 gene, also known as Coq7 is essential for coenzyme Q (ubiquinone, CoQ) synthesis, maintenance of mitochondrial integrity, and neurogenesis in mice. Homozygous mutant mice exhibit developmental arrest and die at E10.5. This mutant strain is useful for the study of Clk1 gene function.
Colony maintenance	Sibling Mating (Heterozygote x Wild-type, Heterozygote)Homozygous mutant mice die at E10.5.
References	Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans. Takahashi M, Asaumi S, Honda S, Suzuki Y, Nakai D, Kuroyanagi H, Shimizu T, Honda Y, Shirasawa T Biochem. Biophys. Res. Commun., 286, 534-540 (2001). 11511092 Mouse homologue of coq7/clk-1, longevity gene in Caenorhabditis elegans, is essential for coenzyme Q synthesis, maintenance of mitochondrial integrity, and neurogenesis. Nakai D, Yuasa S, Takahashi M, Shimizu T, Asaumi S, Isono K, Takao T, Suzuki Y, Kuroyanagi H, Hirokawa K, Koseki H, Shirsawa T Biochem. Biophys. Res. Commun., 289, 463-471 (2001). 11716496 Transgenic expression of the EXT2 gene in developing chondrocytes enhances the synthesis of heparan sulfate and bone formation in mice. Morimoto K, Shimizu T, Furukawa K, Morio H, Kurosawa H, Shirasawa T Biochem. Biophys. Res. Commun., 292, 999-1009 (2002). 11944914

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Coq7	demethyl-Q 7	7	Coq7	targeted mutation 1, Takuji Shirasawa			primary coenzyme Q10 deficiency 8(MedGEN)
neo	neomycin resistance gene (E. coli)	7				herpes simplex virus thymidine kinase promoter (HSV tk promoter)	primary coenzyme Q10 deficiency 8(MedGEN)

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal aerobic respiration(MP:0010957) abnormal embryonic neuroepithelial layer differentiation(MP:0000786) abnormal lysosome morphology(MP:0005058) abnormal mitochondrial morphology(MP:0006035) decreased cell proliferation(MP:0000352) more 3 phenotypes decreased embryo size(MP:0001698) embryonic lethality during organogenesis, complete penetrance(MP:0011098) open neural tube(MP:0000929)
Detailed phenotype data

Ordering Information
Donor DNA	mouse clk1 genomic DNA, E.coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research application
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Takuji Shirasawa (Tokyo Metropolitan Institute of Gerontology)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese), Lab HP (English) Genotyping protocol -PCR-

BRC mice in Publications

No Data