RBRC00467

Information1

Image
BRC No.RBRC00467
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Coq7<tm1Tsh>/ShsRbrc
Former Common nameClk-1 Knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearanceunfixed
Strain developmentDeveloped by Dr. Takuji Shirasawa at Tokyo Metropolitan Institute of Gerontology in 2001. A neomycin selection cassette was inserted into the exon 2 of the Clk1 gene. 129, C57BL/6 and ICR mixed background.
Strain descriptionThe Clk1 gene, also known as Coq7 is essential for coenzyme Q (ubiquinone, CoQ) synthesis, maintenance of mitochondrial integrity, and neurogenesis in mice. Homozygous mutant mice exhibit developmental arrest and die at E10.5. This mutant strain is useful for the study of Clk1 gene function.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote)Homozygous mutant mice die at E10.5.
ReferencesBiochem. Biophys. Res. Commun., 292, 999-1009 (2002). 11944914
Biochem. Biophys. Res. Commun., 289, 463-471 (2001). 11716496
Biochem. Biophys. Res. Commun., 286, 534-540 (2001). 11511092

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Coq7demethyl-Q 77Coq7<tm1Tsh>targeted mutation 1, Takuji Shirasawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse clk1 genomic DNA, E.coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakuji Shirasawa (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data