RBRC00465

Information1

Image
BRC No.RBRC00465
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Ext2,LacZ)E22Tsh/ShsRbrc
Former Common nameTg-EXT2(E22), EXT2 transgenic mice (E22)
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. K. Morimoto and T. Shirasawa at Tokyo Metropolitan Institute of Gerontology in 2001. The Ext2/lacZ plasmid was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionExt2, exostoses (multiple) 2 encodes an essential component of the glycosyltransferase complex that is required for the biosynthesis of heparan sulfate. This transgenic mice express exogenous Ext2 in developing chondrocytes. The expression of Ext2 is histologically monitored by lacZ expression. The biosynthesis of heparan sulfate and the formation of trabeculae of Tg mice are significantly increased at 8 weeks of age. This strain is useful for studies of biosynthesis of heparan sulfate in vivo.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6J)
ReferencesBiochem. Biophys. Res. Commun., 292, 999-1009 (2002). 11944914
Biochem. Biophys. Res. Commun., 286, 534-540 (2001). 11511092

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ext2exostoses (multiple) 2UNExt2mouse Col11a2 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNmouse Col11a2 promoter

Information2

Donor DNAmouse EXT2 cDNA, mouse collagen 11a2 promoter region (-742〜+380), SV40 poly A, E. coli lacZ
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakuji Shirasawa (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data