RBRC00462

Information1

Image
BRC No.RBRC00462
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mak<tm1Yshk>/YshkRbrc
Former Common nameMAK Knockout mice B6
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Shinkai at Kyoto University. A neo cassette was transferred into TT2 ES cells to replace exons 5-8 in the Mak gene. The mutant mice were backcrossed to C57BL/6NJcl for 9 times.
Strain descriptionB6.Cg-Mak<tm1Yshk>. Mak, male germ cell-associated kinase gene expresses specifically in testicular germ cells. Mak deficient mice show no gross abnormalities, and most of the mice were fertile. However, the litter size derived homozygous mutant males and their sperm motility in vitro were mildly reduced compared to those of normal mice. This strain is useful for elucidation of the function of Mak gene in spermatogenesis.
Colony maintenanceSibling mating.Homozygous male are fertile, but exhibit slight reduction in litter size and sperm motility in vitro.
ReferencesMol. Cell. Biol., 22, 3276-3280 (2002). 11971961

Health Report

Examination Date / Room / Rack2014/08/18Room:3-CRack:F
2014/06/16Room:3-CRack:F
2014/04/21Room:3-CRack:F
2014/02/18Room:3-CRack:F
2013/12/16Room:3-CRack:F
2013/10/21Room:3-CRack:F
2013/08/26Room:3-CRack:F
2013/07/02Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Makmale germ cell-associated kinase13Mak<tm1Yshk>targeted mutation 1, Yoichi Shinkai

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse male germ cell-associated kinase (MAK) genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCell Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorFrances Ford (Kamakura Research LaboratoriesRoche)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Omori Y, Chaya T, Katoh K, Kajimura N, Sato S, Muraoka K, Ueno S, Koyasu T, Kondo M, Furukawa T.
Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival.
Proc. Natl. Acad. Sci. U.S.A. 107(52) 22671-6(2010) 21148103