Strain Data Sheet

RBRC00458

Information1

Image
BRC No.RBRC00458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cdkn1b<tm1Kin>
Former Common nameB6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Keiko Nakayama and Kei-ichi Nakayama, Howard Hughes Medical Institute, Washington University School of Medicine in 1996. E14 ES cells were used to generate the mutant mice. The mutant mice were backcrossed to C57BL/6.
Strain descriptionp27 (Cdkn1b) gene knockout mice. A 2.5 kb region containing exons 1 and 2 was replaced with a PGK-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdkn1bcyclin-dependent kinase inhibitor 1B6Cdkn1b<tm1Kin>targeted mutation 1, Kei-ichi NakayamaKip1, p27, p27Kip1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), mouse PGK polyA, mouse Cdkn1b genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data