Strain Data Sheet

RBRC00457

Strain Information

Image
BRC No.RBRC00457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prkcd<tm1Kin>
Former Common namePKCδ Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Keiichi Nakayama, Kyusyu University.
Strain descriptionProtein kinase C (PKC) delta is a member of PKC family which phosphorylates a wide variety of protein targets and is known to be involved in diverse cellular signaling pathways. In PKC family members, PKC delta has a unique function to supress cell proliferation. PKC-delta deficient mice exhibit expansion of the B- lymphocyte population and show autoimmune disease after 6 months of age.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta.
Miyamoto A, Nakayama K, Imaki H, Hirose S, Jiang Y, Abe M, Tsukiyama T, Nagahama H, Ohno S, Hatakeyama S, Nakayama K I
Nature, 416, 865-869 (2002). 11976687

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prkcdprotein kinase C, delta14Prkcdtargeted mutation 1, Kei-ichi Nakayama
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell proliferation(MP:0005153)

  • abnormal Peyer's patch germinal center morphology(MP:0002391)

  • abnormal bone ossification(MP:0008271)

  • abnormal immunoglobulin level(MP:0002490)

  • abnormal lymph node germinal center morphology(MP:0008522)
    • more 31 phenotypes
  • abnormal osteoblast differentiation(MP:0008395)

  • abnormal pancreatic beta cell morphology(MP:0005217)

  • abnormal plasma cell differentiation(MP:0008099)

  • arrested B cell differentiation(MP:0001802)

  • decreased B-1 B cell number(MP:0004978)

  • decreased B-2 B cell number(MP:0008207)

  • decreased body weight(MP:0001262)

  • decreased follicular B cell number(MP:0008174)

  • decreased insulin secretion(MP:0003059)

  • decreased marginal zone B cell number(MP:0008182)

  • decreased width of hypertrophic chondrocyte zone(MP:0003409)

  • enlarged lymph nodes(MP:0000702)

  • enlarged spleen(MP:0000691)

  • glomerulonephritis(MP:0002743)

  • impaired glucose tolerance(MP:0005293)

  • increased B cell apoptosis(MP:0008782)

  • increased B cell number(MP:0005014)

  • increased B cell proliferation(MP:0005154)

  • increased IgA level(MP:0002495)

  • increased IgG1 level(MP:0008499)

  • increased IgG2b level(MP:0008501)

  • increased IgG3 level(MP:0008502)

  • increased IgM level(MP:0002494)

  • increased acute inflammation(MP:0005088)

  • increased anti-chromatin antibody level(MP:0004829)

  • increased follicular B cell number(MP:0008173)

  • increased interleukin-6 secretion(MP:0008705)

  • increased marginal zone B cell number(MP:0008181)

  • increased spleen germinal center number(MP:0008481)

  • increased transitional stage B cell number(MP:0008189)

  • renal glomerular immunoglobulin deposits(MP:0020519)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Prkcd genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Chichger H, Grinnell KL, Casserly B, Chung CS, Braza J, Lomas-Neira J, Ayala A, Rounds S, Klinger JR, Harrington EO.
    Genetic disruption of protein kinase Cδ reduces endotoxin-induced lung injury.
    Am J Physiol Lung Cell Mol Physiol 303(10) L880-8(2012) 22983354