RBRC00457

Information1

Image
BRC No.RBRC00457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prkcd<tm1Kin>
Former Common namePKCδ Knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Keiichi Nakayama, Kyusyu University.
Strain descriptionProtein kinase C (PKC) delta is a member of PKC family which phosphorylates a wide variety of protein targets and is known to be involved in diverse cellular signaling pathways. In PKC family members, PKC delta has a unique function to supress cell proliferation. PKC-delta deficient mice exhibit expansion of the B- lymphocyte population and show autoimmune disease after 6 months of age.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesNature, 416, 865-869 (2002). 11976687

Health Report

Examination Date / Room / Rack2007/11/12Room:4-ARack:B
2007/09/18Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Prkcdprotein kinase C, delta14Prkcd<tm1Kin>targeted mutation 1, Kei-ichi Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Prkcd genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Chichger H, Grinnell KL, Casserly B, Chung CS, Braza J, Lomas-Neira J, Ayala A, Rounds S, Klinger JR, Harrington EO.
Genetic disruption of protein kinase C delta reduces endotoxin-induced lung injury.
Am. J. Physiol. Lung Cell Mol. Physiol. 303(10) L880-8(2012) 22983354