Strain Data Sheet

RBRC00455

Strain Information

Image
BRC No.RBRC00455
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Skp2<tm1Kin>
Former Common nameSkp2 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kei-ichi Nakayama, Kyusyu University in 2000.
Strain descriptionB6.129P2-Skp2<tm1Kin>. Skp2 gene knock-out mice. Homozygous mutant mice are viable, but show reduced growth rate and increased apoptosis of cells.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication.
Nakayama K, Nagahama H, Minamishima Y A, Matsumoto M, Nakamichi I, Kitagawa K, Shirane M, Tsunematsu R, Tsukiyama T, Ishida N, Kitagawa M, Nakayama K, Hatakeyama S
EMBO J., 19, 2069-2081 (2000). 10790373

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Skp2S-phase kinase-associated protein 215Skp2targeted mutation 1, Kei-ichi Nakayama
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal apoptosis(MP:0001648)

  • abnormal cell proliferation(MP:0000350)

  • abnormal hepatocyte morphology(MP:0000607)

  • abnormal immunoglobulin V(D)J recombination(MP:0008755)

  • abnormal kidney morphology(MP:0002135)
    • more 7 phenotypes
  • abnormal testis morphology(MP:0001146)

  • decreased body weight(MP:0001262)

  • delayed wound healing(MP:0002908)

  • increased apoptosis(MP:0006042)

  • increased fibroblast apoptosis(MP:0013501)

  • polyploidy(MP:0004025)

  • postnatal growth retardation(MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Skp2 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data