RBRC00445

Information1

Image
BRC No.RBRC00445
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameC57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The CAG and acr-EGFP plasmids were co-injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6NCr background.
Strain descriptionTransgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc].
ReferencesFEBS Lett., 449, 277-283 (1999). 10338148
Curr. Top Dev. Biol., 44, 1-20 (1999). 9891875
FEBS Lett., 407, 313-319 (1997). 9175875
Dev. Biol., 237, 222-231 (2001). 11518518

Health Report

Examination Date / Room / Rack2012/12/03Room:3-6Rack:D
2012/10/01Room:3-6Rack:D
2012/08/06Room:3-6Rack:D
2012/06/04Room:3-6Rack:D
2012/04/02Room:3-6Rack:D
2012/02/06Room:3-6Rack:D
2011/12/05Room:3-6Rack:D
2011/10/03Room:3-6Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal
Research applicationFluorescent Proteins/lacZ System
Reproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 237, 222-231 (2001). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Genetic Background

BRC mice in Publications

Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
Biochem. Biophys. Res. Commun. (2018) 29705697

Lin YN, Roy A, Yan W, Burns KH, Matzuk MM.
Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis.
Mol. Cell. Biol. 27(19) 6794-805(2007) 17664285

Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T.
Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
Proc. Natl. Acad. Sci. U.S.A. (2012) 22984182