Strain Data Sheet

RBRC00440

Strain Information

Image
BRC No.RBRC00440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gab1<tm1Thir>/ThirRbrc
Former Common nameGab1 Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearancegray
yellow
Strain development
Strain description
Colony maintenance
References
Role of Gab1 in heart, placenta, and skin development and growth factor- and cytokine-induced extracellular signal-regulated kinase mitogen-activated protein kinase activation.
Itoh M, Yoshida Y, Nishida K, Narimatsu M, Hibi M, Hirano T
Mol. Cell. Biol., 20, 3695-3704 (2000). 10779359

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gab1growth factor receptor bound protein 2-associated protein 18Gab1targeted mutation 1, Toshio Hirano
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)
    • IRESinternal ribosomal entry site (EMCV)8
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)
    • SASplice acceptor8SA
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)
    • lacZbeta-galactosidase (E. coli)8
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)
    • neoneomycin resistance gene (E. coli)8
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal epidermis stratum granulosum morphology(MP:0001239)

  • abnormal hair follicle development(MP:0003704)

  • absent eyelids(MP:0001341)

  • decreased embryo size(MP:0001698)

  • decreased trophoblast giant cell number(MP:0001713)
    • more 12 phenotypes
  • dilated heart left ventricle(MP:0002753)

  • dilated heart right ventricle(MP:0002754)

  • early eyelid opening(MP:0003153)

  • hemopericardium(MP:0005244)

  • hemorrhage(MP:0001914)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • petechiae(MP:0008816)

  • placental labyrinth hypoplasia(MP:0001715)

  • small placenta(MP:0004259)

  • thin epidermis(MP:0001218)

  • thin ventricular wall(MP:0000280)

  • ventricular hypoplasia(MP:0000279)
    • Detailed phenotype data

    Ordering Information

    Donor DNASA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Bgeo(E. coli lacZ, E. coli neo), mouse Gab1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 1 year after provided to the RIKEN BRC.
    The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshio Hirano (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data