Strain Data Sheet
RBRC00438
Information1 | |
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| Image |  |
| BRC No. | RBRC00438 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Map2k4<tm1Pngr>/Rbrc |
| Former Common name | SEK1 Knockout mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Hiroshi Nishina at Ontario Cancer Institute, University of Toronto in 1997. A neomycin selection cassette was inserted into the exon 2 of the SEK1 gene. The SEK1 deficient mice were backcrossed to C57BL/6 over 13 times. |
| Strain description | B6.129P-Map2k4<tm1Pngr>. The stress signaling kinase SEK1/MKK4 is a direct activator of stress-activated protein kinase, or Jun N-terminal kinases (SAPKs/JNKs) in response to environmental stress or mitogenic factors. Homozygous mutant mice exhibit severe anemia and die between embryonic day 10.5 and 12.5. This mutant strain is useful for the study of SEK1 gene function. |
| Colony maintenance | Backross to C57BL/6 (Heterozygote x C57BL/6J)Homozygous mutant mice die between embryonic day 10.5 and 12.5. |
| References | Dev. Biol., 250, 332-347 (2002). 12376107 Development, 126, 505-516 (1999). 9876179 Nature, 385, 350-353 (1997). 9002521 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Map2k4mitogen activated protein kinase kinase 411Map2k4<tm1Pngr>targeted mutation 1, Josef M PenningerMEK4, MKK4, Sek1, JNKK1, Serk1, PRKMK4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse mitogen activated protein kinase kinase 4 genomic DNA, E. coli neo |
| Research application | Cell Biology Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hiroshi Nishina (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S. Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production. Biochem Biophys Res Commun 410(2) 301-6(2011) 21664343 Sugiyama D, Kulkeaw K, Mizuochi C. TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts. Mech Dev 130(2-3) 195-206(2013) 23041440 |