Strain Data Sheet

RBRC00438

Strain Information

Image
BRC No.RBRC00438
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Map2k4<tm1Pngr>/Rbrc
Former Common nameSEK1 Knockout mice
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hiroshi Nishina at Ontario Cancer Institute, University of Toronto in 1997. A neomycin selection cassette was inserted into the exon 2 of the SEK1 gene. The SEK1 deficient mice were backcrossed to C57BL/6 over 13 times.
Strain descriptionB6.129P-Map2k4<tm1Pngr>. The stress signaling kinase SEK1/MKK4 is a direct activator of stress-activated protein kinase, or Jun N-terminal kinases (SAPKs/JNKs) in response to environmental stress or mitogenic factors. Homozygous mutant mice exhibit severe anemia and die between embryonic day 10.5 and 12.5. This mutant strain is useful for the study of SEK1 gene function.
Colony maintenanceBackross to C57BL/6 (Heterozygote x C57BL/6J)Homozygous mutant mice die between embryonic day 10.5 and 12.5.
References
SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis.
Watanabe T, Nakagawa K, Ohata S, Kitagawa D, Nishitai G, Seo J, Tanemura S, Shimizu N, Kishimoto H, Wada T, Aoki J, Arai H, Iwatsubo T, Mochita M, Watanabe T, Satake M, Ito Y, Matsuyama T, Mak T W, Penninger J M, Nishina H, Katada T
Dev. Biol., 250, 332-347 (2002). 12376107

Defective liver formation and liver cell apoptosis in mice lacking the stress signaling kinase SEK1/MKK4.
Nishina H, Vaz C, Billia P, Nghiem M, Sasaki T, De la Pompa J L, Furlonger K, Paige C, Hui C, Fischer K D, Kishimoto H, Iwatsubo T, Katada T, Woodgett J R, Penninger J M
Development, 126, 505-516 (1999). 9876179

Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3.
Nishina H, Fischer K D, Radvanyi L, Shahinian A, Hakem R, Rubie E A, Bernstein A, Mak T W, Woodgett J R, Penninger J M
Nature, 385, 350-353 (1997). 9002521

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Map2k4mitogen-activated protein kinase kinase 411Map2k4targeted mutation 1, Josef M Penninger
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal liver development(MP:0000596)

  • anemia(MP:0001577)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mitogen activated protein kinase kinase 4 genomic DNA, E. coli neo
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHiroshi Nishina (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S.
    Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production.
    Biochem Biophys Res Commun 410(2) 301-6(2011) 21664343
    Sugiyama D, Kulkeaw K, Mizuochi C.
    TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts.
    Mech Dev 130(2-3) 195-206(2013) 23041440