RBRC00437

Information1

Image
BRC No.RBRC00437
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prnp<tm1Ito>/Rbrc
Former Common namePrnp-/- mouse, Prion protein (PrP) Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Takashi Yokoyama, National Institute of Animal Health and Shigeyoshi Itohara, RIKEN BSI in 2001.C57BL/6J background.
Strain descriptionPrP, the prion protein, plays a central role in the pathogenesis of transmissible neurodegenerative disorders such as scrapie or BSE. Homozygous mutant mice show the absence of cellular isoform of prion protein. Aged homozygous mice exhibit tremor and ataxia.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Aged mice exhibit tremor and ataxia.
ReferencesJ. Biol. Chem., 276, 11265-11271 (2001). 11152682
Nature, 400, 225-226 (1999). 10421360

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
PrnPprion protein2PrnP<tm1Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse prion genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationMouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 1999 Jul 15;400(6741):225-6.
DepositorTakashi Onodera (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H.
Cutting Edge: Brucella abortus Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor.
J. Immunol. 189(4) 1540-4(2012) 22772447