RBRC00408

Information1

Image
BRC No.RBRC00408
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gfap<tm1Ito>/ItoRbrc
Former Common nameGfap KO, GFAP-KO-clone 27
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1995. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionGfap knockout mice. Immediately downstream of the endogenous start codon of Gfap was replaced with a cassette, containing a promoterless lacZ and pgk-neo. Gfap, glial fibrillary acidic protein is an intermediate filament protein specifically expressed in astrocytes. Homozygous mutant mice develop normally and not show any detectable abnormalities in the anatomy of the brain. But, exhibit a impairment of long-term depression (LTD) and PF-Purkinje cell synapses and eyeblink, suggested that the Gfap is required for communications between Bergmann glia and Purkinje cells.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
ReferencesNeuron, 14, 29-41 (1995). 7826639

Health Report

Examination Date / Room / Rack2009/10/19Room:3-DRack:G
2009/08/24Room:3-DRack:G
2009/06/22Room:3-DRack:G
2009/04/21Room:3-DRack:G
2009/04/20Room:3-DRack:G
2009/02/23Room:3-DRack:G
2008/12/15Room:3-DRack:G
2008/10/20Room:3-DRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gfapglial fibrillary acidic protein11Gfap<tm1Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)11endogenous start codon

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse glial fibrillary acidic protein genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Gomi et al., Neuron, 14, 29-41 (1995).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.
Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP.
Genes Dev. 22(4) 489-98(2008) 18281462