Strain Data Sheet

RBRC00398

update : 2023-09-20

Information1
Image
BRC No.	RBRC00398
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129P2-Mmp2<tm1Ito>/ItoRbrc
Former Common name	GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype
ES Cell line	E14 [129P2/OlaHsd]
Background strain	C57BL/6JJcl
Appearance	black
Strain development	Developed by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.
Strain description	This strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Colony maintenance	Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
References	J. Biol. Chem., 272, 22389-22392 (1997). 9278386

Health Report
Examination Date / Room / Rack	2023/07/10Room:3-ARack:B 2023/04/10Room:3-ARack:B 2023/01/10Room:3-ARack:B 2022/10/11Room:3-ARack:B 2022/09/05Room:3-ARack:B 2022/07/11Room:3-ARack:B 2022/04/11Room:3-ARack:B 2022/03/28Room:3-ARack:B 2022/01/24Room:3-ARack:B 2021/10/26Room:3-ARack:B

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2
Donor DNA	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA
Research application	Cardiovascular Research Developmental Biology Research
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 272, 22389-22392 (1997).
Depositor	Shigeyoshi Itohara (RIKEN)
Strain Status	Live mice Frozen embryos Frozen sperm
Strain Availability	Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months)
Additional Info.	Genetic Background Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S. Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts. Am J Pathol 173(3) 781-91(2008) 18669618 Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H. Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy. Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097 Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A. Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction. Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562 Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T. Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging. Contrast Media Mol Imaging 2019 8908943(2019) 30723388 Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE. Matrix metalloproteinase 14 is required for fibrous tissue expansion. Elife 4 e09345(2015) 26390284 Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM. Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis. J Exp Med 203(4) 1007-19(2006) 16585265 Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H. Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2. Kidney Int 78(1) 69-78(2010) 20375993 Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S. Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice. Nephrol Dial Transplant 28(1) 55-62(2013) 23028104 Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N. Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway. Redox Biol 4 169-79(2015) 25574848

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts.
Am J Pathol 173(3) 781-91(2008) 18669618

Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H.
Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy.
Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097

Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A.
Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction.
Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562

Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T.
Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging.
Contrast Media Mol Imaging 2019 8908943(2019) 30723388

Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.
Matrix metalloproteinase 14 is required for fibrous tissue expansion.
Elife 4 e09345(2015) 26390284

Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.
J Exp Med 203(4) 1007-19(2006) 16585265

Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2.
Kidney Int 78(1) 69-78(2010) 20375993

Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice.
Nephrol Dial Transplant 28(1) 55-62(2013) 23028104

Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N.
Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.
Redox Biol 4 169-79(2015) 25574848

Information1

Health Report

Gene

Information2

BRC mice in Publications