Strain Data Sheet

RBRC00398

Information1

Image
BRC No.RBRC00398
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp2<tm1Ito>/ItoRbrc
Former Common nameGelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.
Strain descriptionThis strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Colony maintenanceHomozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
ReferencesJ. Biol. Chem., 272, 22389-22392 (1997). 9278386

Health Report

Examination Date / Room / Rack2022/04/11Room:3-ARack:B
2022/03/28Room:3-ARack:B
2022/01/24Room:3-ARack:B
2021/10/26Room:3-ARack:B
2021/07/26Room:3-ARack:B
2021/04/26Room:3-ARack:B
2021/02/01Room:3-ARack:B
2020/11/30Room:3-ARack:B
2020/11/09Room:3-ARack:B
2020/08/24Room:3-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA
Research applicationCardiovascular Research
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 272, 22389-22392 (1997).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts.
Am J Pathol 173(3) 781-91(2008) 18669618

Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H.
Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy.
Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097

Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A.
Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction.
Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562

Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T.
Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging.
Contrast Media Mol Imaging 2019 8908943(2019) 30723388

Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.
Matrix metalloproteinase 14 is required for fibrous tissue expansion.
Elife 4 e09345(2015) 26390284

Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.
J Exp Med 203(4) 1007-19(2006) 16585265

Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2.
Kidney Int 78(1) 69-78(2010) 20375993

Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice.
Nephrol Dial Transplant 28(1) 55-62(2013) 23028104

Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N.
Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.
Redox Biol 4 169-79(2015) 25574848