RBRC00398

Information1

Image
BRC No.RBRC00398
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp2<tm1Ito>/ItoRbrc
Former Common nameGelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.
Strain descriptionThis strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Colony maintenanceHomozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
ReferencesJ. Biol. Chem., 272, 22389-22392 (1997). 9278386

Health Report

Examination Date / Room / Rack2020/02/17Room:3-ARack:B
2019/12/16Room:3-ARack:B
2019/10/21Room:3-ARack:B
2019/08/26Room:3-ARack:B
2019/06/17Room:3-ARack:B
2019/04/15Room:3-ARack:B
2019/02/18Room:3-ARack:B
2019/01/28Room:3-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA
Research applicationCardiovascular Research
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 272, 22389-22392 (1997).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts.
Am. J. Pathol. 173(3) 781-91(2008) 18669618

Hidenori MATSUSAKA, Masaki IKEUCHI, Shouji MATSUSHIMA, Tomomi IDE, Toru KUBOTA, Arthur M FELDMAN, Akira TAKESHITA, Kenji SUNAGAWA, Hiroyuki TSUTSUI
Selective Disruption of Matrix Metalloproteinase-2 Gene Exacerbates Myocardial Inflammation and Dysfunction in Mice with Cytokine-Induced Cardiomyopathy.
Am. J. Physiol. Heart Circ. Physiol. 289 H1858-1864(2005) 15937097

Shunji HAYASHIDANI, Hiroyuki TSUTSUI, Masaki IKEUCHI, Tetsuya SHIOMI, Hidenori MATSUSAKA, Toru KUBOTA, Kyoko IMANAKA-YOSHIDA, Takeshi ITOH, Akira TAKESHITA
Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction.
Am. J. Physiol. Heart Circ. Physiol. 285 H1229-1235(2003) 12775562

Taylor SH, Yeung CC, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.
Matrix metalloproteinase 14 is required for fibrous tissue expansion.
Elife (2015) 26390284

Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.
J. Exp. Med. 203(4) 1007-19(2006) 16585265

Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2.
Kidney Int. 78(1) 69-78(2010) 20375993

Takamiya Y, Fukami K, Yamagishi SI, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice.
Nephrol. Dial. Transplant. (2012) 23028104

Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, N gre-Salvayre A, Aug N.
Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.
Redox Biol 4C 169-179(2014) 25574848