RBRC00361

Information1

Image
BRC No.RBRC00361
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC57BL/6-TgH(p38)/118
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6J
Appearanceblack [a/a]
Strain developmentThis B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.
Strain descriptionActivity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression.
Colony maintenanceThe mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14).
ReferencesCell, 102, 221-231(2000). 10943842
J. Biol. Chem., 277, 37896-37903 (2002). 12138127

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mapk14mitogen activated protein kinase 1417Mapk14<tm1Mka>targeted mutation 1, Michael Karin

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17

Information2

Donor DNAE. coli neo, mouse p38 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTatsuhiko Sudo (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Miki OHTA, Keisuke TATEISHI, Fumihiko KANAI, Satoshi UEHA, Bayasi GULENG, Miwa WASHIDA, Yasuo TANAKA, Hideaki IJICHI, Tsuneo IKENOUE, Masataka SATA, Tatsuhiko SUDO, Shuichiro SHIINA, Takao KAWABE, Kouji MATSUSHIMA, Masao OMATA
Reduced p38 mitogen-activated protein kinase in donor grafts accelerates acute intestinal graft-versus-host disease in mice.
Eur. J. Immunol. 35 2210-2221(2005) 15971269