RBRC00306

Information1

Image
BRC No.RBRC00306
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameTNF.K.O
Former Common name
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Michael W Marion and Lloyd J. Old, Ludwig Institute for Cancer Research in 1999. Neomycin cassettes were inserted into the nucleotides 3704-5364 of the Tnf gene.
Strain descriptionB6.129S1-Tnf<tm1Ljo>/Rbrc. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine, play a critical role in regulating of the development and function of the immune system. Homozygous mutant mice show no cytotoxicity of TNF.
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice show a normal development and no gross morphological abnormalities.
ReferencesProc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tnftumor necrosis factor17Tnf<tm1Ljo>targeted mutation 1, Lioyd J Old

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse tumor necrosis factor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Inquiries regarding the use of the BIOLOGICAL RESOURCE for commercial purposes including research use by for-profit companies should be directed to:
Dr. Par Olsson
Director, Intellectual Property
Ludwig Institute for Cancer Research
666 Third Avenue
New York, NY 10017
Tel. +1-212-450-1500
Fax. +1-212-450-1535

Ownership of modifications and derivatives including crossed progeny of the BIOLOGICAL RESOURCE will be determined in accordance with applicable law governing inventorship and the creative input of the parties and the Recipient agrees to report any such progeny to LICR. The RIKEN BRC, the LICR and Recipient agree that no party will license or otherwise commercialize any such invention in the absence of an agreement to be negotiated in good faith by the parties hereto, providing for, inter alia, the sharing of royalty income.
DepositorGerd Ritter (Ludwig Institute for Cancer Research)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data