Strain Data Sheet
RBRC00305
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC00305 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc |
| Former Common name | C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B |
| H-2 Haplotype | |
| ES Cell line | W9.5/D3 for TNF alpha, CCE [129/Sv] for relA |
| Background strain | C57BL/6 |
| Appearance | black [a/a B/B C/C] |
| Strain development | Produced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997. |
| Strain description | TNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo. |
| Colony maintenance | TNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type |
| References | Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality. Doi T S, Marino M W, Takahashi T, Yoshida T, Sakakura T, Old L J, Obata Y Proc. Natl. Acad. Sci. USA, 96, 2994-2999 (1999). 10077625Characterization of tumor necrosis factor-deficient mice. Marino M W, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old L J Proc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses. Doi T S, Takahashi T, Taguchi O, Azuma T, Obata Y J. Exp. Med., 185, 953-961 (1997). 9120401 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Tnf | tumor necrosis factor | 17 | Tnf | targeted mutation 1, Lioyd J Old | more 1 Diseases | ||
| neo | neomycin resistance gene (E. coli) | 17 | mouse phosphoglycerate kinase promoter (PGK promoter) | more 1 Diseases | |||
| Rela | v-rel reticuloendotheliosis viral oncogene homolog A (avian) | 19 | Rela | targeted mutation 1, Yuichi Obata | more 1 Diseases | ||
| neo | neomycin resistance gene (E. coli) | 19 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | more 1 Diseases | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | more 47 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tnf genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse Rela genomic DNA |
| Research application | Apoptosis Research Cancer Research Cardiovascular Research Developmental Biology Research Diabetes and Obesity Research Endocrine Deficiency Research Hematological Research Immunology and Inflammation Research Internal/Organ Research Neurobiology Research covid19_onset covid19_progression |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 96, 2994-2999 (1999). The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100 Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice. |
| Depositor | Takahiro Doi (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2009 |
BRC mice in Publications |
|---|
| No Data |