Information1 | |
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Image | ![]() |
BRC No. | RBRC00267 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb |
Former Common name | C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131 |
H-2 Haplotype | |
ES Cell line | |
Background strain | Inter- Substrain hybrid between C57BL/6Cr Slc and C57BL/6J Jcl |
Appearance | black [a/a] |
Strain development | Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 background (C57BL/6JJcl and C57BL/6NCrSlc mixed). |
Strain description | These transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Homozygous transgenic females experienced show a high incidence of sudden death (about 30%), putatively due to dilated cardiomyopaty. Transgene integration site: 14D1. |
Colony maintenance | Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc] |
References | Mol. Reprod. Dev., 54, 43-8 (1999). 10423296 Genomics, 80, 564-574 (2002). 12504848 Curr. Top Dev. Biol., 44, 1-20 (1999). 9891875 FEBS Lett., 407, 313-319 (1997). 9175875 |
Health Report | |
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Examination Date / Room / Rack | 2023/08/28Room:4-BRack:G 2023/05/29Room:4-BRack:G 2023/02/27Room:4-BRack:G 2022/11/29Room:4-BRack:G 2022/08/29Room:4-BRack:G 2022/05/31Room:4-BRack:G 2022/03/07Room:4-BRack:G 2021/12/06Room:4-BRack:G |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Information2 | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA |
Research application | Cell Biology Research Developmental Biology Research Fluorescent Proteins/lacZ System Reproductive Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 407, 313-319 (1997). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Mouse of the Month Feb 2012 Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung. Biol Reprod 87(2) 42(2012) 22674387 Matoba S, Ogura A. Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice. Biol Reprod 84(4) 631-8(2011) 21076079 Fujiki Y, Johnson KL, Peter I, Tighiouart H, Bianchi DW. Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers. Biol Reprod 81(1) 26-32(2009) 19279322 Ohmura M, Ogawa T, Ono M, Dezawa M, Hosaka M, Kubota Y, Sawada H. Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal. Biol Reprod 68(6) 2304-13(2003) 12606404 Ogawa T, Ohmura M, Yumura Y, Sawada H, Kubota Y. Expansion of murine spermatogonial stem cells through serial transplantation. Biol Reprod 68(1) 316-22(2003) 12493728 Fujihara Y, Takato T, Hoshi K. Immunological response to tissue-engineered cartilage derived from auricular chondrocytes and a PLLA scaffold in transgenic mice. Biomaterials 31(6) 1227-34(2010) 19913296 Tsujigiwa H, Nishizaki K, Teshima T, Takeda Y, Yoshinobu J, Takeuchi A, Orita Y, Sugata Y, Nagatsuka H, Nagai N. The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium. Brain Res 1052(1) 10-5(2005) 15996641 Muramatsu M, Yamamoto S, Osawa T, Shibuya M. Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth. Cancer Res 70(20) 8211-21(2010) 20924106 Joo SY, Cho KA, Jung YJ, Kim HS, Park SY, Choi YB, Hong KM, Woo SY, Seoh JY, Ryu KH. Bioimaging for the monitoring of the in vivo distribution of infused mesenchymal stem cells in a mouse model of the graft-versus-host reaction. Cell Biol Int 35(4) 417-21(2011) 21062262 Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH. Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow. Cell Biol Int 33(7) 772-7(2009) 19427913 Ju SY, Cho KA, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH. Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver. Cell Biol Int 33(1) 31-5(2009) 18992833 Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Mimura O, Taguchi A, Matsuyama T. Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke. Cell Death Differ 19(5) 756-67(2012) 22052192 Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S. Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell 14(2) 217-27(2014) 24506885 Pritchard S, Peter I, Johnson KL, Bianchi DW. The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon. Chimerism 3(3) 59-64(2012) 23128065 Jeon HJ, Choi JH, Jung IH, Park JG, Lee MR, Lee MN, Kim B, Yoo JY, Jeong SJ, Kim DY, Park JE, Park HY, Kwack K, Choi BK, Kwon BS, Oh GT. CD137 (4-1BB) deficiency reduces atherosclerosis in hyperlipidemic mice. Circulation 121(9) 1124-33(2010) 20176988 Kiguchi N, Kobayashi Y, Saika F, Kishioka S. Epigenetic upregulation of CCL2 and CCL3 via histone modifications in infiltrating macrophages after peripheral nerve injury. Cytokine 64(3) 666-72(2013) 24135048 Aoki H, Yamada Y, Hara A, Kunisada T. Two distinct types of mouse melanocyte: differential signaling requirement for the maintenance of non-cutaneous and dermal versus epidermal melanocytes. Development 136(15) 2511-21(2009) 19553284 Choi JB, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H. Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta cells. Diabetologia 46(10) 1366-74(2003) 12898006 Hiratsuka S, Tomita T, Mishima T, Matsunaga Y, Omori T, Ishibashi S, Yamaguchi S, Hosogane T, Watarai H, Omori-Miyake M, Yamamoto T, Shibata N, Watanabe A, Aburatani H, Tomura M, High KA, Maru Y. Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung. EMBO Mol Med 10(7) (2018) 29930175 Kawase Y, Yanagi Y, Takato T, Fujimoto M, Okochi H. Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth. Exp Cell Res 295(1) 194-203(2004) 15051502 Unno A, Kachi S, Batanova TA, Ohno T, Elhawary N, Kitoh K, Takashima Y. Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver. Exp Parasitol 134(2) 160-4(2013) 23538031 Shimizukawa R, Sakata A, Hirose M, Takahashi A, Iseki H, Liu Y, Kunita S, Sugiyama F, Yagami K. Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously. Genesis 42(1) 47-52(2005) 15830376 Inoue S, Ishikawa K, Nakada K, Sato A, Miyoshi H, Hayashi J. Suppression of disease phenotypes of adult mito-mice carrying pathogenic mtDNA by bone marrow transplantation. Hum Mol Genet 15(11) 1801-7(2006) 16613898 Hirata T, Osuga Y, Yoshino O, Hirota Y, Harada M, Takemura Y, Morimoto C, Koga K, Yano T, Tsutsumi O, Taketani Y. Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein. Hum Reprod 20(8) 2092-6(2005) 15831509 Nishio N, Ito S, Suzuki H, Isobe K. Antibodies to wounded tissue enhance cutaneous wound healing. Immunology 128(3) 369-80(2009) 20067537 Tomida D, Nishiguchi KM, Kataoka K, Yasuma TR, Iwata E, Uetani R, Kachi S, Terasaki H. Suppression of choroidal neovascularization and quantitative and qualitative inhibition of VEGF and CCL2 by heparin. Invest Ophthalmol Vis Sci 52(6) 3193-9(2011) 21296829 Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H. The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization. Invest Ophthalmol Vis Sci 52(3) 1431-8(2011) 21051720 Kobayashi Y, Kiguchi N, Fukazawa Y, Saika F, Maeda T, Kishioka S. Macrophage-T cell interactions mediate neuropathic pain through the glucocorticoid-induced tumor necrosis factor ligand system. J Biol Chem 290(20) 12603-13(2015) 25787078 Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A. Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer. J Cell Sci 119(Pt 10) 1985-91(2006) 16621892 Sato A, Toyoshima KE, Toki H, Ishibashi N, Asakawa K, Iwadate A, Kanayama T, Tobe H, Takeda A, Tsuji T. Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection. J Dermatol 39(8) 682-7(2012) 22380609 Osada A, Kobayashi K, Masui S, Hamazaki TS, Yasuda K, Okochi H. Cloned cells from the murine dermal papilla have hair-inducing ability. J Dermatol Sci 54(2) 129-31(2009) 19150224 Nishida T, Tsuji S, Tsujii M, Ishii S, Yoshio T, Shinzaki S, Egawa S, Irie T, Kakiuchi Y, Yasumaru M, Iijima H, Tsutsui S, Kawano S, Hayashi N. Cultured bone marrow cell local implantation accelerates healing of ulcers in mice. J Gastroenterol 43(2) 124-35(2008) 18306986 Oura R, Arakaki R, Yamada A, Kudo Y, Tanaka E, Hayashi Y, Ishimaru N. Induction of rapid T cell death and phagocytic activity by Fas-deficient lpr macrophages. J Immunol 190(2) 578-85(2013) 23255359 Kiguchi N, Kobayashi Y, Kadowaki Y, Fukazawa Y, Saika F, Kishioka S. Vascular endothelial growth factor signaling in injured nerves underlies peripheral sensitization in neuropathic pain. J Neurochem 129(1) 169-78(2014) 24304382 Liu N, Deguchi K, Yamashita T, Liu W, Ikeda Y, Abe K. Intracerebral transplantation of bone marrow stromal cells ameliorates tissue plasminogen activator-induced brain damage after cerebral ischemia in mice detected by in vivo and ex vivo optical imaging. J Neurosci Res 90(11) 2086-93(2012) 22791305 Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano HJ, Kawakami Y, Toyama Y, Toda M. Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery. J Neurosci Res 76(4) 453-65(2004) 15114617 Ishigaki H, Yamamoto Y, Ishida H, Kajino K, Itoh Y, Fujiyama Y, Ogasawara K. Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice. Microbiol Immunol 50(8) 655-62(2006) 16924151 Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T. Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ. Nat Commun 4 2497(2013) 24084941 Toyoshima KE, Asakawa K, Ishibashi N, Toki H, Ogawa M, Hasegawa T, Irié T, Tachikawa T, Sato A, Takeda A, Tsuji T. Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches. Nat Commun 3 784(2012) 22510689 Nakao K, Morita R, Saji Y, Ishida K, Tomita Y, Ogawa M, Saitoh M, Tomooka Y, Tsuji T. The development of a bioengineered organ germ method. Nat Methods 4(3) 227-30(2007) 17322892 Ashina K, Tsubosaka Y, Nakamura T, Omori K, Kobayashi K, Hori M, Ozaki H, Murata T. Histamine Induces Vascular Hyperpermeability by Increasing Blood Flow and Endothelial Barrier Disruption In Vivo. PLoS One 10(7) e0132367(2015) 26158531 Vogelgesang A, Scapin C, Barone C, Tam E, Blumental Perry A, Dammann CE. Cigarette smoke exposure during pregnancy alters fetomaternal cell trafficking leading to retention of microchimeric cells in the maternal lung. PLoS One 9(5) e88285(2014) 24832066 Baba M, Kitoh K, Takashima Y. Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture. Parasitol Int 65(5 Pt B) 536-538(2016) 26806654 Unno A, Suzuki K, Xuan X, Nishikawa Y, Kitoh K, Takashima Y. Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow. Parasitol Int 57(4) 515-8(2008) 18652914 Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, Ogawa M, Mizuno M, Kasugai S, Tsuji T. Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A 106(32) 13475-80(2009) 19666587 Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A. Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells. Proc Natl Acad Sci U S A 104(30) 12389-94(2007) 17636128 González-González E, López-Casas PP, Del Mazo J. Gene silencing by RNAi in mouse Sertoli cells. Reprod Biol Endocrinol 6 29(2008) 18620581 Asakawa K, Toyoshima KE, Ishibashi N, Tobe H, Iwadate A, Kanayama T, Hasegawa T, Nakao K, Toki H, Noguchi S, Ogawa M, Sato A, Tsuji T. Hair organ regeneration via the bioengineered hair follicular unit transplantation. Sci Rep 2 424(2012) 22645640 Nakano-Doi A, Nakagomi T, Fujikawa M, Nakagomi N, Kubo S, Lu S, Yoshikawa H, Soma T, Taguchi A, Matsuyama T. Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction. Stem Cells 28(7) 1292-302(2010) 20517983 |