BRC No.RBRC00267
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb
Former Common nameC57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131
H-2 Haplotype
ES Cell line
Background strainInter- Substrain hybrid between C57BL/6Cr Slc and C57BL/6J Jcl
Appearanceblack [a/a]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 background (C57BL/6JJcl and C57BL/6NCrSlc mixed).
Strain descriptionThese transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Homozygous transgenic females experienced show a high incidence of sudden death (about 30%), putatively due to dilated cardiomyopaty. Transgene integration site: 14D1.
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc]
ReferencesMol. Reprod. Dev., 54, 43-8 (1999). 10423296
FEBS Lett., 407, 313-319 (1997). 9175875
Genomics, 80, 564-574 (2002). 12504848
Curr. Top Dev. Biol., 44, 1-20 (1999). 9891875

Health Report

Examination Date / Room / Rack2020/03/30Room:4-BRack:G


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
Research applicationCell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Reproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 407, 313-319 (1997). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Mouse of the Month Feb 2012
Genetic Background

BRC mice in Publications

Tada M, Shinohara Y, Kato I, Hiraga K, Aizawa T, Demura M, Mori Y, Shinoda H, Mizuguchi M, Kawano K.
Preparation and observation of fresh-frozen sections of the green fluorescent protein transgenic mouse head.
Acta Histochem Cytochem 39(2) 9952(2006) 17375207

Ooka H, Kanda S, Okazaki H, Suzuki H, Mishima K, Saito I, Yagi M, Tomoda K, Nishiyama T.
Characterization of side population (SP) cells in murine cochlear nucleus.
Acta Otolaryngol. (2012) 22667338

Kageyama S, Liu H, Nagata M, Aoki F.
The role of ETS transcription factors in transcription and development of mouse preimplantation embryos.
Biochem. Biophys. Res. Commun. 344(2) 675-9(2006) 16630543

Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification of a Persistent Stem Cell Type.
Biol. Reprod. (2016) 27122635

Pritchard S, Wick HC, Slonim DK, Johnson KL, Bianchi DW.
Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung.
Biol. Reprod. 87(2) 42(2012) 22674387

Matoba S, Ogura A.
Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice.
Biol. Reprod. 84(4) 631-8(2011) 21076079

Fujiki Y, Johnson KL, Peter I, Tighiouart H, Bianchi DW.
Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers.
Biol. Reprod. 81(1) 26-32(2009) 19279322

Masako OHMURA, Takehiko OGAWA, Michio ONO, Mari DEZAWA, Masahiko HOSAKA, Yoshinobu KUBOTA, Hajime SAWADA
Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal.
Biol. Reprod. 68 2304-2313(2003) 12606404

Takehiko OGAWA, Masako OHMURA, Yasushi YUMURA, Hajime SAWADA, Yoshinobu KUBOTA
Expansion of murine spermatogonial stem cells through serial transplantation.
Biol. Reprod. 68 316-322(2003) 12493728

Fujihara Y, Takato T, Hoshi K.
Immunological response to tissue-engineered cartilage derived from auricular chondrocytes and a PLLA scaffold in transgenic mice.
Biomaterials 31(6) 1227-34(2010) 19913296

Hidetsugu TSUJIGIWA, Kazunori NISHIZAKI, Takanori TESHIMA, Yasushi TAKEDA, Junko YOSHINOBU, Ayako TAKEUCHI, Yorihisa ORITA, Yuji SUGATA, Hitoshi NAGATSUKA, Noriyuki NAGAI
The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium.
Brain Res. 1052(1) 10-15(2005) 15996641

Muramatsu M, Yamamoto S, Osawa T, Shibuya M.
Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth.
Cancer Res. 70(20) 8211-21(2010) 20924106

Joo SY, Cho KA, Jung YJ, Kim HS, Park SY, Choi YB, Hong KM, Woo SY, Seoh JY, Ryu KH.
Bioimaging for the monitoring of the in vivo distribution of infused mesenchymal stem cells in a mouse model of the graft-versus-host reaction.
Cell Biol. Int. 35(4) 417-21(2011) 21062262

Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
Cell Biol. Int. 33(7) 772-7(2009) 19427913

Ju SY, Cho KA, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
Cell Biol. Int. 33(1) 40693(2009) 18992833

Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Mimura O, Taguchi A, Matsuyama T.
Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke.
Cell Death Differ. (2011) 22052192

Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.
Cell Stem Cell 14(2) 217-27(2014) 24506885

Pritchard S, Peter I, Johnson KL, Bianchi DW.
The natural history of fetal cells in postpartum murine maternal lung and bone marrow: A two-stage phenomenon.
Chimerism 3(3) (2012) 23128065

Jeon HJ, Choi JH, Jung IH, Park JG, Lee MR, Lee MN, Kim B, Yoo JY, Jeong SJ, Kim DY, Park JE, Park HY, Kwack K, Choi BK, Kwon BS, Oh GT.
CD137 (4-1BB) deficiency reduces atherosclerosis in hyperlipidemic mice.
Circulation 121(9) 1124-33(2010) 20176988

Kiguchi N, Kobayashi Y, Saika F, Kishioka S.
Epigenetic upregulation of CCL2 and CCL3 via histone modifications in infiltrating macrophages after peripheral nerve injury.
Cytokine (2013) 24135048

Aoki H, Yamada Y, Hara A, Kunisada T.
Two distinct types of mouse melanocyte: differential signaling requirement for the maintenance of non-cutaneous and dermal versus epidermal melanocytes.
Development 136(15) 2511-21(2009) 19553284

J B CHOI, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H
Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta cells.
Diabetologia 46 1366-1374(2003) 12898006

Hiratsuka S, Tomita T, Mishima T, Matsunaga Y, Omori T, Ishibashi S, Yamaguchi S, Hosogane T, Watarai H, Omori-Miyake M, Yamamoto T, Shibata N, Watanabe A, Aburatani H, Tomura M, High KA, Maru Y.
Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung.
EMBO Mol Med 10 e8643(2018) 29930175

Kawase Y, Yanagi Y, Takato T, Fujimoto M, Okochi H.
Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth.
Exp. Cell Res. 295 194-203(2004) 15051502

Unno A, Kachi S, Batanova TA, Ohno T, Elhawary N, Kitoh K, Takashima Y.
Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver.
Exp. Parasitol. (2013) 23538031

Rie SHIMIZUKAWA, Aya SAKATA, Michiko HIROSE, Akio TAKAHASHI, Hiroyoshi ISEKI, Ying LIU, Satoshi KUNITA, Fumihiro SUGIYAMA, Ken-ichi YAGAMI
Establishment of a New Embryonic Stem Cell Line Derived From C57BL/6 Mouse Expressing EGFP Ubiquitously
Genesis 42 47-52(2005) 15830376

Inoue S, Ishikawa K, Nakada K, Sato A, Miyoshi H, Hayashi J.
Suppression of disease phenotypes of adult mito-mice carrying pathogenic mtDNA by bone marrow transplantation.
Hum. Mol. Genet. 15(11) 1801-7(2006) 16613898

Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.
Hum. Reprod. 20 2092-2096(2005) 15831509

Nishio N, Ito S, Suzuki H, Isobe K.
Antibodies to wounded tissue enhance cutaneous wound healing.
Immunology 128(3) 369-80(2009) 20067537

Tomida D, Nishiguchi KM, Kataoka K, Yasuma TR, Iwata E, Uetani R, Kachi S, Terasaki H.
Suppression of Choroidal Neovascularization and Quantitative and Qualitative Inhibition of VEGF and CCL2 by Heparin.
Invest. Ophthalmol. Vis. Sci. 52(6) 471042(2011) 21296829

Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H.
The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization.
Invest. Ophthalmol. Vis. Sci. 52(3) 1431-8(2011) 21051720

Kobayashi Y, Kiguchi N, Fukazawa Y, Saika F, Maeda T, Kishioka S.
Macrophage-T cell interactions mediate neuropathic pain through the glucocorticoid-induced tumor necrosis factor ligand system.
J. Biol. Chem. 290(20) 12603-12613(2015) 25787078

Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A.
Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer.
J. Cell. Sci. 119(Pt 10) 1985-91(2006) 16621892

Sato A, Toyoshima KE, Toki H, Ishibashi N, Asakawa K, Iwadate A, Kanayama T, Tobe H, Takeda A, Tsuji T.
Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection.
J. Dermatol. (2012) 22380609

Osada A, Kobayashi K, Masui S, Hamazaki TS, Yasuda K, Okochi H.
Cloned cells from the murine dermal papilla have hair-inducing ability.
J. Dermatol. Sci. 54(2) 129-31(2009) 19150224

Nishida T, Tsuji S, Tsujii M, Ishii S, Yoshio T, Shinzaki S, Egawa S, Irie T, Kakiuchi Y, Yasumaru M, Iijima H, Tsutsui S, Kawano S, Hayashi N.
Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.
J. Gastroenterol. 43(2) 124-35(2008) 18306986

Oura R, Arakaki R, Yamada A, Kudo Y, Tanaka E, Hayashi Y, Ishimaru N.
Induction of Rapid T Cell Death and Phagocytic Activity by Fas-Deficient lpr Macrophages.
J. Immunol. (2012) 23255359

Kiguchi N, Kobayashi Y, Kadowaki Y, Fukazawa Y, Saika F, Kishioka S.
Vascular endothelial growth factor signaling in injured nerves underlies peripheral sensitization in neuropathic pain.
J. Neurochem. (2013) 24304382

Liu N, Deguchi K, Yamashita T, Liu W, Ikeda Y, Abe K.
Intracerebral transplantation of bone marrow stromal cells ameliorates tissue plasminogen activator-induced brain damage after cerebral ischemia in mice detected by in vivo and ex vivo optical imaging.
J. Neurosci. Res. (2012) 22791305

Yuji MIKAMI, Hideyuki OKANO, Masanori SAKAGUCHI, Masaya NAKAMURA, Takuya SHIMAZAKI, Hirotaka James OKANO, Yutaka KAWAKAMI, Yoshiaki TOYAMA, Masahiro TODA
Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery.
J. Neurosci. Res. 76 453-465(2004) 15114617

Ishigaki H, Yamamoto Y, Ishida H, Kajino K, Itoh Y, Fujiyama Y, Ogasawara K.
Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
Microbiol. Immunol. 50(8) 655-62(2006) 16924151

Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T.
Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ.
Nat Commun 4 2497(2013) 24084941

Toyoshima KE, Asakawa K, Ishibashi N, Toki H, Ogawa M, Hasegawa T, Irie T, Tachikawa T, Sato A, Takeda A, Tsuji T.
Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches.
Nat Commun 3 784(2012) 22510689

Nakao K, Morita R, Saji Y, Ishida K, Tomita Y, Ogawa M, Saitoh M, Tomooka Y, Tsuji T.
The development of a bioengineered organ germ method.
Nat. Methods 4(3) 227-30(2007) 17322892

Ashina K, Tsubosaka Y, Nakamura T, Omori K, Kobayashi K, Hori M, Ozaki H, Murata T.
Histamine Induces Vascular Hyperpermeability by Increasing Blood Flow and Endothelial Barrier Disruption In Vivo.
PLoS ONE (2015) 26158531

Vogelgesang A, Scapin C, Barone C, Tam E, Blumental Perry A, Dammann CE.
Cigarette Smoke Exposure during Pregnancy Alters Fetomaternal Cell Trafficking Leading to Retention of Microchimeric Cells in the Maternal Lung.
PLoS ONE 9(5) e88285 e88285(2014) 24832066

Baba M, Kitoh K, Takashima Y.
Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture.
Parasitol. Int. (2016) 26806654

Unno A, Suzuki K, Xuan X, Nishikawa Y, Kitoh K, Takashima Y.
Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow.
Parasitol. Int. 57(4) 515-8(2008) 18652914

Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, Ogawa M, Mizuno M, Kasugai S, Tsuji T.
Fully functional bioengineered tooth replacement as an organ replacement therapy.
Proc. Natl. Acad. Sci. U.S.A. 106(32) 13475-80(2009) 19666587

Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.
Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells.
Proc. Natl. Acad. Sci. U.S.A. 104(30) 12389-94(2007) 17636128

Gonz lez-Gonz lez E, L pez-Casas PP, Del Mazo J.
Gene silencing by RNAi in mouse Sertoli cells.
Reprod. Biol. Endocrinol. 6 29(2008) 18620581

Asakawa K, Toyoshima KE, Ishibashi N, Tobe H, Iwadate A, Kanayama T, Hasegawa T, Nakao K, Toki H, Noguchi S, Ogawa M, Sato A, Tsuji T.
Hair organ regeneration via the bioengineered hair follicular unit transplantation.
Sci Rep 2 424(2012) 22645640

Nakano-Doi A, Nakagomi T, Fujikawa M, Nakagomi N, Kubo S, Lu S, Yoshikawa H, Soma T, Taguchi A, Matsuyama T.
Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction.
Stem Cells 28(7) 1292-302(2010) 20517983