RBRC00254

Information1

Image
BRC No.RBRC00254
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameC57BL/6-TgN(a-CaMKII-nlCre)/20
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Futatsugi A. The Cre-Strain series were transfered to Experimental Animal Division, RIKEN Tsukuba Institute in 1999.
Strain descriptionThe Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre in each line of Tg mice was following order; Line 10, 26> 22, 17N > 17A, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitativeone), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines.
Colony maintenanceMaintained by sister-brother mating. Breed well, Hemizygote x Wild-type, or hemizygote hemizygote.
References

Health Report

Examination Date / Room / Rack2008/10/06Room:3-4Rack:D
2008/06/09Room:3-4Rack:D
2008/04/08Room:3-4Rack:D
2008/04/07Room:3-4Rack:D
2008/02/04Room:3-4Rack:D
2007/12/03Room:3-4Rack:D
2007/10/01Room:3-4Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcremouse Camk2a promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNAmouse a-CaMKII promoter, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase DNA, poly A
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall prior contact with the DEPOSITOR, discussn use of it and obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and NOT for commercial use. The RECIPIENT can use the BIOLOGICAL RESOURCE in a collaboration basis with the DEPOSITOR, except for some cases where only ACKNOLEGEMENT is requested by citing the DEPOSITOR. BEFORE the publication on the BIOLOGICAL RESOURCE by the DEPOSITOR, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR and add Drs. Akira Futatsugi and Katsuhiko Mikoshiba as a co-authors. The RECIPIENT agrees to cite publications requested by the DEPOSITOR.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Iwano T, Masuda A, Kiyonari H, Enomoto H, Matsuzaki F.
Prox1 postmitotically defines dentate gyrus cells by specifying granule cell identity over CA3 pyramidal cell fate in the hippocampus.
Development 139(16) 3051-62(2012) 22791897

Yabe D, Fukuda H, Aoki M, Yamada S, Takebayashi S, Shinkura R, Yamamoto N, Honjo T.
Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP.
Genesis 45(5) 300-6(2007) 17457934