RBRC00235

Information1

Image
BRC No.RBRC00235
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129X1-Chrng<tm1Mtak>/10Rbrc
Former Common nameC57BL/6-TgH(AchR-gamma/10)
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Dr. Masazumi Takahashi. This strain has been introduced into C57BL/6 genetic background by successive backcrossing for more than 10 generations in RIKEN Tsukuba Institute.
Strain descriptionThe acetylcholine receptors are known to have important roles in neuro-muscular functions. The gamma subunit are expressed in embryonic stages and the epsilon subunit are expressed postnatal stages.The acetylcholine receptors gamma and epsilon subunits have been disrupted by homologous recombination to study the functions of these genes and switching mechanism of gamma to epsilon subunits. The gamma subunit deficient homozygotes died after two days post delivery, suffering from inability of motor function of the limbs and drinking milk. The analysis on the neuro-muscular junctions showed the structural and electrophysiological abnormalities.
Colony maintenanceBackcrossing to C57BL/6. Or sister-brother mating of heterozygotes. Breeding performance is good.
ReferencesProc. Natl. Acad. Sci. USA, 95, 13413-13418 (1998). 9811814
FEBS Lett., 223, 104-112 (1987). 3666131
EMBO Rep., 3, 674-681 (2002). 12101101
Proc. Natl. Acad. Sci. USA, 93, 13286-13291 (1996). 8917583
Development, 124, 5075-5086 (1997). 9362465

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Chrngcholinergic receptor, nicotinic, gamma polypeptide1Chrng<tm1Mtak>targeted mutation 1, Masazumi Takahashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse muscle type nicotinic acetylcholine receptor gamma subunit
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMasazumi Takahashi (Centre National de Genotypage)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Liu Y, Padgett D, Takahashi M, Li H, Sayeed A, Teichert RW, Olivera BM, McArdle JJ, Green WN, Lin W.
Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning.
Development 135(11) 1957-67(2008) 18434415