BRC No.RBRC00169
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameC57BL/6N-TgH(Tnc), B6-TN, TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NJcl
Appearanceblack [a/a, B/B, C/C]
Strain developmentCongenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N.
Strain descriptionTenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice are viable and fertile.
ReferencesExp. Mol. Pathol., 72, 186-195 (2002). 12009782
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
Genes Dev., 6, 1821-1831 (1992). 1383086
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
J. Neurosci., 15, 1971-1983 (1995). 7534342
Am. J. Pathol., 152, 1237-1245 (1998). 9588892
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
Gene, 104, 177-185 (1991). 1717349
Jpn. J. Cancer Res., 93, 968-975 (2002). 12359049
J. Comp. Neurol., 430, 485-500 (2001). 11169482

Health Report

Examination Date / Room / Rack2020/06/01Room:3-CRack:K


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAE. coli lacZ, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tenascin genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999).
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y.
Extracellular matrix protein Tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
Blood (2012) 22553313

The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse.
Differentiation 71 291-298(2003) 12823230

Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H.
Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C.
Jpn. J. Cancer Res. 93 968-975(2002) 12359049

Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T.
Integrin alpha 9 on lymphatic endothelial cells regulates lymphocyte egress.
Proc. Natl. Acad. Sci. U.S.A. 111(8) 429647(2014) 24516133