BRC No.RBRC00159
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-p53 tm1Sia-Tg(gpt Delta)
Former Common nameC57BL/6-TgH(p53)-TgN(gpt delta)
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain developmentThe original p53 deficient mice had been introduced into C57BL/6 background for 13 generations by Dr. Niwa T at Hiroshima Univ (-1995). Then the mice were transfered to Dr. Kusakabe M. at RIKEN Life Science Center for further backcrossing to establish the B6 congenic strain. The gpt delta mice were crossed with C57BL/6-p53tm1 to establish a double mutant at RIKEN, and have been maintained by sister-brother mating for 8 generations.
Strain description
Colony maintenance
ReferencesCarcinogenesis, 21, 2049-2056 (2000). 11062167
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728
Cancer Lett., 143, 241-244 (1999). 10503911
Phys. Med. 17 Suppl. 1:192-193, (2001). 11776258
Oncogene, 8, 3313-3322 (1993). 7504233
Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718
Cancer Res., 61, 3913-3918 (2001). 11358805
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079
Environ. Mutagen Res., 22, 85-90 (2000).

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trp53transformation-related protein 5311Trp53<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
gpt Deltaguanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>transgene insertion 1, Takehito Nohmi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
red/gamlambda phage red/gam17red/gam


Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA, lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
Research applicationApoptosis Research
Cancer Research
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
Toxicology Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

Sakai H, Tsukamoto T, Yamamoto M, Shirai N, Iidaka T, Hirata A, Yanai T, Masegi T, Donehower LA, Tatematsu M.
High susceptibility of nullizygous p53 knockout mice to colorectal tumor induction by 1,2-dimethylhydrazine.
J. Cancer Res. Clin. Oncol. 129 335-340(2003) 12743811

Ken-ichi MASUMURA, Mieko HORIGUCHI, Akiyoshi NISHIKAWA, Takashi UMEMURA, Keita KANKI, Yusuke KANKE, Takehiko NOHMI
Low dose genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in gpt delta transgenic mice.
Mutat. Res. 541 91-102(2003) 14568298

Akiyoshi NISHIKAWA, Takayoshi IMAZAWA, Yuichi KUROIWA, Yasuki KITAMURA, Keita KANKI, Yuji ISHII, Takashi UMEMURA, Masao HIROSE
Induction of colon tumors in C57BL/6J mice fed MeIQx, IQ, or PhIP followed by dextran sulfate sodium treatment.
Toxicol. Sci. 84 243-248(2005) 15635146