RBRC00158

Information1

Image
BRC No.RBRC00158
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-TgN(gpt Delta)
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain developmentThis strain was developed for efficient detection of mutations in vivo by Dr.Nohmi T.
Strain descriptionThis strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718
Cancer Lett., 143, 241-244 (1999). 10503911
Cancer Res., 61, 3913-3918 (2001). 11358805
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717
Carcinogenesis, 21, 2049-2056 (2000). 11062167
Environ. Mutagen Res., 22, 85-90 (2000).

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
gpt Deltaguanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>transgene insertion 1, Takehito Nohmi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
red/gamlambda phage red/gam17red/gam

Information2

Donor DNAlambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorTakehiko Noumi (National Institute of Health Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data