RBRC00153

Information1

Image
BRC No.RBRC00153
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-cre)10Kmik/KmikRbrc
Former Common nameC57BL/6-TgN(a-CaMKII-nlCre)/10
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeposited by Dr. Katsuhiko Mikoshiba, Brain Science Institute, RIKEN.
Strain descriptionThe Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre gene in each line of Tg mice was following order; Line 10, 26>22, 17N, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitative one), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines.
Colony maintenanceSibling mating (Hemizygote x Wild-type)Reproductive perdormance is good.
References

Health Report

Examination Date / Room / Rack2015/11/09Room:4-ARack:G
2015/03/23Room:4-ARack:G
2015/01/19Room:4-ARack:G
2014/11/17Room:4-ARack:G
2014/09/16Room:4-ARack:G
2014/07/14Room:4-ARack:G
2014/05/19Room:4-ARack:G
2014/05/14Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcremouse Camk2a promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNAmouse a-CaMKII promoter, phage P1 Cre recombinase DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), poly A
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall prior contact with the DEPOSITOR, discussn use of it and obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and NOT for commercial use. The RECIPIENT can use the BIOLOGICAL RESOURCE in a collaboration basis with the DEPOSITOR, except for some cases where only ACKNOLEGEMENT is requested by citing the DEPOSITOR. BEFORE the publication on the BIOLOGICAL RESOURCE by the DEPOSITOR, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR and add Drs. Akira Futatsugi and Katsuhiko Mikoshiba as a co-authors. The RECIPIENT agrees to cite publications requested by the DEPOSITOR.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Shioda N, Moriguchi S, Oya T, Ishii Y, Shen J, Matsushima T, Nishijo H, Sasahara M, Fukunaga K.
Aberrant hippocampal spine morphology and impaired memory formation in neuronal platelet-derived growth factor beta-receptor lacking mice.
Hippocampus (2011) 21997856