RBRC00109

Information1

Image
BRC No.RBRC00109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc
Former Common nameC3H/HeN-TgH(Zic1), Zic1 KO (C3H background)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae](D3 subline)
Background strainC3H/HeNJcl
Appearanceagouti
Strain developmentDeveloped by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations.
Strain descriptionThe Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.Genotype of Affected Mutation:Zic1 null heterozygous.
Colony maintenanceBackcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs.
ReferencesMol. Brain Res., 78, 15-25 (2000). 10891581
J. Neurosci., 18, 284-293 (1998). 9412507
Dev. Biol., 244, 329-341 (2002). 11944941
Mech. Dev., 89, 141-150 (1999). 10559489

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zic1zinc finger protein of the cerebellum 19Zic1<tm1Jaru>targeted mutation 1, Jun Aruga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Zic1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationDevelopmental Biology Research
Mouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 18, 284-293 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorJun Aruga (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP

BRC mice in Publications

No Data