RBRC00107

Information1

Image
BRC No.RBRC00107
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC3.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameC3H/HeN-TgH(p53)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC3H/HeNCrlCrlj
Appearanceagouti
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj]
ReferencesOncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trp53transformation-related protein 5311Trp53<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
Research applicationCancer Research
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorOhtsura Niwa (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Jun 2005

BRC mice in Publications

Sugihara T, Murano H, Tanaka K, Oghiso Y.
Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells.
J. Radiat. Res. 49(3) 231-40(2008) 18285661

Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
Activation of Interferon-Stimulated Genes by {gamma}-Ray Irradiation Independently of the Ataxia Telangiectasia Mutated-p53 Pathway.
Mol. Cancer Res. 9(4) 476-84(2011) 21357441

Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
p53-Mediated Gene Activation in Mice at High Doses of Chronic Low-Dose-Rate gamma Radiation.
Radiat Res. (2010) 21189076

Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation.
Radiat. Res. 175(3) 328-35(2011) 21388276