BRC No.RBRC00067
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameBALB/cA-TgH(Tnc), BALB/c-TN, TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAJcl
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. M. Kusakabe. Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN in 1990's. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to BALB/cA.
Strain descriptionC.B6CB-Tnc<tm1Sia>. Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the feild of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction, etc. The tenascin C deficient congenic strains were developed successive by backcrossing into A (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice are viable and fertile.
ReferencesInvest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Am. J. Pathol., 152, 1237-1245 (1998). 9588892
Genes Dev., 6, 1821-1831 (1992). 1383086
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782
J. Comp. Neurol., 430, 485-500 (2001). 11169482
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
J. Neurosci., 15, 1971-1983 (1995). 7534342
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
Gene, 104, 177-185 (1991). 1717349
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
乳癌基礎研究, 8, 26-31 (1999).
炎症, 15(5), 377-381 (1996).
乳癌基礎研究, 5, 41-45 (1996).

Health Report

Examination Date / Room / Rack2018/10/15Room:3-CRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAE. coli lacZ, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tenascin genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Vis. Sci., 40, 1071-1080 (1999).
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Kubo Y, Arima M, Nakao S, Hisatomi T, Ikeda Y, Matsuda A, Sonoda KH, Ishibashi T.
Tenascin-C secreted by transdifferentiated retinal pigment epithelial cells promotes choroidal neovascularization via integrin αV.
Lab. Invest. (2016) 27668890