Strain Data Sheet

RBRC00067

Information1

Image
BRC No.RBRC00067
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameBALB/cA-TgH(Tnc), BALB/c-TN, TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAJcl
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. M. Kusakabe. Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN in 1990's. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to BALB/cA.
Strain descriptionC.B6CB-Tnc<tm1Sia>. Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the feild of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction, etc. The tenascin C deficient congenic strains were developed successive by backcrossing into A (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice are viable and fertile.
ReferencesBiochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
J. Neurosci., 15, 1971-1983 (1995). 7534342
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Gene, 104, 177-185 (1991). 1717349
Genes Dev., 6, 1821-1831 (1992). 1383086
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782
J. Comp. Neurol., 430, 485-500 (2001). 11169482
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
Am. J. Pathol., 152, 1237-1245 (1998). 9588892
炎症, 15(5), 377-381 (1996).
乳癌基礎研究, 5, 41-45 (1996).
乳癌基礎研究, 8, 26-31 (1999).

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi AizawaTnc; tenascin C, Hxb, Ten, TN-C, AI528729

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Vis. Sci., 40, 1071-1080 (1999).
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Kubo Y, Arima M, Nakao S, Hisatomi T, Ikeda Y, Matsuda A, Sonoda KH, Ishibashi T.
Tenascin-C secreted by transdifferentiated retinal pigment epithelial cells promotes choroidal neovascularization via integrin αV.
Lab Invest 96(11) 1178-1188(2016) 27668890